The ultrastructure of MCF-10A acini
The ultrastructure of MCF-10A acini
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DOI：
10.1002/jcp.20639
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发表时间：
2006-07-01
影响因子：
5.6
通讯作者：
Nickerson, Jeffrey A.
中科院分区：
文献类型：
--
作者：
Underwood, Jean M.;Imbalzano, Karen M.;Nickerson, Jeffrey A.
MCF-10A人乳腺上皮细胞在重建的基底膜内培养形成类似于乳腺小叶的腺泡结构的腺泡。这种三维培养系统已被用于识别和表征控制细胞增殖和死亡的信号转导途径，并用于研究恶性进展中的失调。我们比较了MCF-10A腺泡、单层生长的MCF-10A细胞和人乳腺小叶腺泡结构的超微结构。MCF-10A腺泡的组织结构由连接到基底膜的半桥粒和腺泡细胞之间丰富的桥粒形成。中间丝加入到大而丰富的丝束连接半桥粒和桥粒在核表面的网站。在单层MCF-10A细胞中观察到更少和更细的细丝束，在乳腺组织中甚至更少。在乳腺组织中观察到细胞之间的紧密连接，但在NACF-10A腺泡中缺失。MCF-10A腺泡细胞的细胞质具有类似于在乳腺组织中观察到的极性组织，在核的顶侧具有中心体和高尔基体。MCF-10A腺泡细胞核表面不规则，常内陷，单个核仁。异染色质的分布与乳腺组织上皮细胞相似。单层MCF-10A细胞的细胞核具有多个核仁，轮廓更规则，异染色质较少。电子显微镜具有调查MCF-10A细胞和腺泡结构特征所需的分辨率，这些特征可能会随着旨在诱导恶性表型的操作而改变。
MCF-10A human mammary epithelial cells cultured inside reconstituted basement membrane form acini that resemble the acinar Structures of mammary lobules. This three-dimensional Culture system has been used for identifying and characterizing the signal transduction pathways controlling cell proliferation and death, and for studying their disregulation in malignant progression. We have compared the ultrastructure of MCF-10A acini, MCF-10A cells grown in monolayer, and the acinar structures of human breast lobules. The tissue architecture of MCF-10A acini was formed by hemidesmosomes connected to a basement membrane and by abundant desmosomes between acinar cells. Intermediate filaments that joined into large and abundant filament bundles connected hemidesmosomes and desmosomes to sites at the nuclear surface. Fewer and thinner bundles of filaments were observed in monolayer MCF-10A cells and even fewer in breast tissue. Tight junctions were observed between cells in breast tissue but missing in NACF-10A acini. The cytoplasm of MCF-10A acinar cells had a polar organization similar to that observed in breast tissue, with centrosomes and the Golgi apparatus on the apical side of the nucleus. MCF-10A acinar nuclei had an irregular, frequently invaginated Surface and had a single nucleolus. The distribution of heterochromatin was similar to that in the epithelial cells of breast tissue. The nuclei of monolayer MCF-10A cells had multiple nucleoli, a more regular profile, and less heterochromatin. Electron microscopy has the resolution required to Survey features of MCF-10A cell and acinus architecture that may change with manipulations designed to induce malignant phenotypes.