Astrocytic contributions to blood-brain barrier (BBB) formation by endothelial cells: A possible use of aortic endothelial cell for in vitro BBB model
Astrocytic contributions to blood-brain barrier (BBB) formation by endothelial cells: A possible use of aortic endothelial cell for in vitro BBB model
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DOI：
10.1016/0197-0186(95)00142-5
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发表时间：
1996-05-01
影响因子：
4.2
通讯作者：
Kato, T
中科院分区：
文献类型：
--
作者：
Isobe, I;Watanabe, T;Kato, T
在两种血脑屏障（BBB）模型中，利用双室系统共培养法研究了星形胶质细胞对内皮细胞单层通透性的影响：大鼠星形胶质细胞与牛主动脉内皮细胞（BAECs）或牛脑内皮细胞（BBECs）共培养。在系统1中，星形胶质细胞与内皮细胞分离，通过与星形胶质细胞共培养，观察到BBEC单层的L - 葡萄糖通透性降低了40%，但BAEC单层没有变化。尽管培养中的BBEC经过多次传代后，形态从纺锤形转变为鹅卵石样，但在系统1的共培养中，传代后的BBEC对星形胶质细胞仍有反应（L - 葡萄糖通透性降低37%）。相比之下，在系统2中，内皮细胞和星形胶质细胞分别位于膜的上下表面，通过与星形胶质细胞共培养，BAEC和BBEC单层的通透性均降低（BAEC降低75%，BBEC降低40%）。在这种与星形胶质细胞连续共培养（系统2）的情况下，BAEC显示出许多体内血脑屏障特有的紧密连接样结构。这些结果表明，在体内经星形胶质细胞诱导的原代培养BBEC可能通过一种或多种星形胶质细胞可溶性因子，对星形胶质细胞保持较高的敏感性，从而表现出血脑屏障特异性表型。并且即使是来自神经外组织的BAEC，当在体外与星形胶质细胞紧密共培养时，也可能获得类似的表型，可用于体外血脑屏障模型的广泛应用。版权所有（C）1996爱思唯尔科学有限公司
Astrocytic contribution of endothelial cell monolayer permeability was examined in two blood-brain barrier (BBB) models, using the coculture in a double chamber system: rat astrocytes and bovine aortic endothelial cells (BAECs) or bovine brain endothelial cells (BBECs). In system 1, where astrocytes were separated from endothelial cells, a 40% reduction in L-glucose permeability of the BBEC monolayer, but not the BAEC monolayer, was observed by cocultivation with astrocytes. Although several passages of BBEC in culture elicited morphological transformation from spindle-shapes to cobblestone-like features, the passaged BBECs remained responsive to astrocytes in coculture in system 1 (37% reduction of the L-glucose permeability). By contrast, in system 2, where respective endothelial cells and astrocytes layered on the upper and lower surfaces of a membrane, the permeability of both BAEC and BBEC monolayers was reduced by cocultivation with astrocytes (75% reduction for BAEC and 40% reduction for BBEC). BAECs in this contiguous coculture (system 2) with astrocytes showed numerous tight junction-like structures characteristic of the BBB in rico. These results suggest that primary cultured BBECs, which had been primed by astrocytes in oivo, retain a higher sensitivity to astrocytes possibly through an astrocytic soluble factor (s) to exhibit BBB-specific phenotypes. and that even BAEC From extra-neural tissues, when cultured with astrocytes in close proximity in vitro, may acquire the similar phenotypes and serve for an extensive use of BBB model in vitro. Copyright (C) 1996 Elsevier Science Ltd.