Molecular evolution of flavonoid dioxygenases in the family Apiaceae
Molecular evolution of flavonoid dioxygenases in the family Apiaceae
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DOI：
10.1016/j.phytochem.2005.03.030
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发表时间：
2005-06-01
期刊：
影响因子：
3.8
通讯作者：
Martens, S
中科院分区：
文献类型：
--
作者：
Gebhardt, Y;Witte, S;Martens, S
已知的山松科植物物种积累的黄酮类化合物主要以黄酮类和黄酮醇的形式存在。3种依赖2-氧戊二酸的双加氧酶、黄酮合酶或黄酮3P-羟基酶和黄酮醇合酶参与这些次生代谢物的生物合成。最近从欧芹叶片中克隆了相应的基因。黄酮合酶I似乎仅存在于菊科植物中，其独特的存在及其与黄烷酮3β-羟基酶的高度序列相似性为进化研究奠定了基础。为了研究这两种酶在整个天南星科植物中的相互关系，本研究利用RT-PCR技术克隆并鉴定了菊属、天葵、八角、八角、胡萝卜等植物的黄酮合酶I或黄酮3β-羟基酶基因，获得了3个额外的合酶基因和3个额外的羟基酶基因。这些序列的分子和系统发育分析与基于形态特征的系统发育相一致，表明黄酮合酶I很可能是由于黄烷酮3β-羟基酶基因的重复，以及在松果科亚科发育过程中的功能变化所致。此外，在两种合成酶的基因组序列中，每种合成酶都有两个内含子，羟基酶中没有内含子。这些结果可能是由于进化后期羟基酶的内含子丢失所致。(C)2005爱思唯尔有限公司。保留所有权利。
Plant species of the family Apiaceac are known to accumulate flavonoids mainly in the form of flavones and flavonols. Three 2-oxoglutarate-dependent dioxygenases, flavone synthase or flavanone 3 P-hydroxylase and flavonol synthase are involved in the biosynthesis of these secondary metabolites. The corresponding genes were cloned recently from parsley (Petroselinum crispum) leaves. Flavone synthase I appears to be confined to the Apiaceae, and the unique occurrence as well as its high sequence similarity to flavanone 3 beta-hydroxylase laid the basis for evolutionary studies. In order to examine the relationship of these two enzymes throughout the Apiaceae, RT-PCR based cloning and functional identification of flavone synthases I or flavanone 3 beta-hydroxylases were accomplished from Ammi majus, Anethum graveolens, Apium graveolens, Pimpinella anisum, Conium maculatum and Daucus carota, yielding three additional synthase and three additional hydroxylase cDNAs. Molecular and phylogenetic analyses of these sequences were compatible with the phylogeny based on morphological characteristics and suggested that flavone synthase I most likely resulted from gene duplication of flavanone 3 beta-hydroxylase, and functional diversification at some point during the development of the apiaceae subfamilies. Furthermore, the genomic sequences from Petroselinum crispum and Daucus carota revealed two introns in each of the synthases and a lack of introns in the hydroxylases. These results might be explained by intron losses from the hydroxylases occurring at a later stage of evolution. (c) 2005 Elsevier Ltd. All rights reserved.