Mutation of neutralizing/antibody-dependent enhancing epitope on spike protein and 7b gene of feline infectious peritonitis virus: Influences of viral replication in monocytes/macrophages and virulence in cats
Mutation of neutralizing/antibody-dependent enhancing epitope on spike protein and 7b gene of feline infectious peritonitis virus: Influences of viral replication in monocytes/macrophages and virulence in cats
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DOI：
10.1016/j.virusres.2010.12.020
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发表时间：
2011-03-01
期刊：
影响因子：
5
通讯作者：
Hohdatsu, Tsutomu
中科院分区：
文献类型：
--
作者：
Takano, Tomomi;Tomiyama, Yoshika;Hohdatsu, Tsutomu
我们先前使用实验室菌株猫传染性腹膜炎病毒（FIPV）79-1146（Kida等人，1999年）。Mar突变株病毒与亲本株FIPV 79-1146相比，其Spike蛋白的中和表位发生了几个氨基酸的突变。我们阐明了用于制备mar突变病毒的MAb也失去了其增强同源mar突变病毒的活性，这强烈表明中和和抗体依赖性增强表位存在于毒株FIPV 79-1146的相同区域中。我们还发现中和表位中的氨基酸突变减少了单核细胞/巨噬细胞中的病毒复制。我们还证明了7 b基因的两个核苷酸的突变或缺失使FIPV 79-1146株的毒力丧失。(C)2011 Elsevier B. V.保留所有权利。
We previously prepared neutralizing monoclonal antibody (MAb)-resistant (mar) mutant viruses using a laboratory strain feline infectious peritonitis virus (FIPV) 79-1146 (Kida et al., 1999). Mar mutant viruses are mutated several amino acids of the neutralizing epitope of Spike protein, compared with the parent strain, FIPV 79-1146. We clarified that MAb used to prepare mar mutant viruses also lost its activity to enhance homologous mar mutant viruses, strongly suggesting that neutralizing and antibody-dependent enhancing epitopes are present in the same region in the strain FIPV 79-1146. We also discovered that amino acid mutation in the neutralizing epitope reduced viral replication in monocytes/macrophages. We also demonstrated that the mutation or deletion of two nucleotides in 7b gene abrogate the virulence of strain FIPV 79-1146. (C) 2011 Elsevier B.V. All rights reserved.