Overexpressing temperature-sensitive dynamin decelerates phototransduction and bundles microtubules in Drosophila photoreceptors.
Overexpressing temperature-sensitive dynamin decelerates phototransduction and bundles microtubules in Drosophila photoreceptors.
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DOI：
10.1523/jneurosci.2873-09.2009
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发表时间：
2009-11-11
期刊：
影响因子：
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通讯作者：
Juusola M
中科院分区：
文献类型：
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作者：
Gonzalez-Bellido PT;Wardill TJ;Kostyleva R;Meinertzhagen IA;Juusola M
shibirets1是果蝇基因的一种温度敏感突变，编码动力蛋白同源物，在升高或限制性温度下阻断囊泡内吞噬，从而阻断突触传递。通过靶向表达Gal4， UAS-shibirets1已被用于解剖神经回路。我们研究了在允许温度（19°C）和限制温度（31°C）下果蝇光感受器中UAS-shibirets1过表达的影响。在19°C时，过表达UAS-shits1导致光传导减慢和神经递质释放减少。这种表型随着深色适应、年龄和白色突变体而加剧。过表达uas - shibiret1的光感受器含有广泛的空泡化线粒体，囊泡数量减少和微管捆绑。免疫电镜显示后者是动力蛋白包被。此外，微管表型并不局限于光感受器，因为层状细胞中的UAS-shibirets1过表达也会捆绑微管。我们得出结论，动力蛋白具有多种功能，这些功能被果蝇光感受器中UAS-shibirets1的过度表达所中断，在先前报道的允许温度下不可逆地破坏了它们的神经通讯。
shibirets1, a temperature-sensitive mutation of the Drosophila gene encoding a Dynamin orthologue, blocks vesicle endocytosis and thus synaptic transmission, at elevated, or restrictive temperatures. By targeted Gal4 expression, UAS-shibirets1 has been used to dissect neuronal circuits. We investigated the effects of UAS-shibirets1 overexpression in Drosophila photoreceptors at permissive (19°C) and restrictive (31°C) temperatures. At 19°C, overexpression of UAS-shits1 causes decelerated phototransduction and reduced neurotransmitter release. This phenotype is exacerbated with dark-adaptation, age and in white mutants. Photoreceptors overexpressing UAS-shibirets1 contain terminals with widespread vacuolated mitochondria, reduced numbers of vesicles and bundled microtubules. Immuno-EM reveals that the latter are dynamin coated. Further, the microtubule phenotype is not restricted to photoreceptors, as UAS-shibirets1 overexpression in lamina cells also bundles microtubules. We conclude that dynamin has multiple functions that are interrupted by UAS-shibirets1 overexpression in Drosophila photoreceptors, destabilizing their neural communication irreversibly at previously reported permissive temperatures.