The effects of milk as a food matrix for polyphenols on the excretion profile of cocoa ( − )-epicatechin metabolites in healthy human subjects
The effects of milk as a food matrix for polyphenols on the excretion profile of cocoa ( − )-epicatechin metabolites in healthy human subjects
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DOI：
10.1017/s0007114508922534
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发表时间：
2008-10
影响因子：
3.6
通讯作者：
E. Roura;C. Andrés-Lacueva;R. Estruch;M. Lourdes Mata Bilbao;M. Izquierdo-Pulido;R. Lamuela-Raventós
中科院分区：
文献类型：
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作者：
E. Roura;C. Andrés-Lacueva;R. Estruch;M. Lourdes Mata Bilbao;M. Izquierdo-Pulido;R. Lamuela-Raventós
不同食物基质对多酚代谢和排泄的影响尚不确定。这项研究的目的是评估牛奶对可可粉中（-）-表儿茶素代谢物排泄的可能影响，在摄入可可粉时，牛奶和不摄入牛奶。21名志愿者以随机交叉设计接受以下三种测试餐，每餐之间间隔1周：（1）250 ml全脂牛奶作为对照;（2）40 g可可粉溶于250 ml全脂牛奶（CC-M）;（3）40 g可可粉溶于250 ml水（CC-W）。在食用前和食用后0-6、6-12和12-24 h期间收集尿液。采用液相色谱-MS法测定（−）-表儿茶素代谢物的排泄。在摄入两种可可饮料（CC-M和CC-W）后排泄的尿液中检测到一种（−）-表儿茶素葡糖苷酸和三种（−）-表儿茶素硫酸盐。结果表明，牛奶不会显著影响尿液中排泄的代谢物总量。然而，观察到代谢产物排泄特征存在差异;葡萄糖醛酸苷和硫酸盐排泄率以及排泄期和基质之间的硫酸化位置存在变化。多酚被消耗的基质会影响它们的代谢和排泄，这可能会影响它们的生物活性。因此，需要更多的研究来评估这些不同的代谢产物对身体的影响。
The effect of different food matrices on the metabolism and excretion of polyphenols is uncertain. The objective of the study was to evaluate the possible effect of milk on the excretion of ( − )-epicatechin metabolites from cocoa powder after its ingestion with and without milk. Twenty-one volunteers received the following three test meals each in a randomised cross–over design with a 1-week interval between meals: (1) 250 ml whole milk as a control; (2) 40 g cocoa powder dissolved in 250 ml whole milk (CC–M); (3) 40 g cocoa powder dissolved in 250 ml water (CC–W). Urine was collected before consumption and during the 0–6, 6–12 and 12–24 h periods after consumption. ( − )-Epicatechin metabolite excretion was measured using liquid chromatography–MS. One ( − )-epicatechin glucuronide and three ( − )-epicatechin sulfates were detected in urine excreted after the intake of the two cocoa beverages (CC–M and CC–W). The results show that milk does not significantly affect the total amount of metabolites excreted in urine. However, differences in metabolite excretion profiles were observed; there were changes in the glucuronide and sulfate excretion rates, and the sulfation position between the period of excretion and the matrix. The matrix in which polyphenols are consumed can affect their metabolism and excretion, and this may affect their biological activity. Thus, more studies are needed to evaluate the effect of these different metabolite profiles on the body.