Expression profile of a clonal insulin-expressing epithelial cell in the thymus.
Expression profile of a clonal insulin-expressing epithelial cell in the thymus.
复制标题
胸腺中表达胰岛素的克隆上皮细胞的表达谱。
DOI：
10.1016/j.molimm.2013.07.015
复制
发表时间：
2013
影响因子：
3.6
通讯作者：
Polychronakos,Constantin
中科院分区：
文献类型：
--
作者：
Levi,Dina;Polychronakos,Constantin
背景1型糖尿病是一种自身免疫性疾病，由胰腺β细胞破坏引起。在这种自身反应性应答中靶向的主要抗原之一是胰岛素。研究表明，胰岛素在胸腺中少量表达，更具体地说，在胸腺髓质上皮细胞（mTEC）中也表达多种其他组织特异性抗原。这种胸腺表达能够维持自身耐受性，并且在预防自身免疫性疾病中是必不可少的。我们的实验室已经建立了一个小鼠mTEC克隆细胞系，特异性表达胰岛素，以更好地了解这种异位表达的胰岛素的调节机制。在这项研究中，我们比较了胰岛素表达细胞系的胰岛素非表达mTEC线全基因组的expression profiling.ResultsThe最重要的区别是过度表达的CD 34在胰岛素表达克隆，证实了实时RT-PCR和流式细胞术。胸腺细胞表达较高水平的CD 34被发现含有较高水平的胰岛素，并在较小程度上，Aire，在胸腺中的自我抗原表达的主调节器。结论CD 34可能是一种特异性的功能性标志物，对胰岛素具有一定的特异性。
BackgroundType 1 diabetes is an autoimmune disease resulting from the destruction of pancreatic beta-cells. One of the main antigens targeted in this auto reactive response is insulin. It has been shown that insulin is expressed in small amounts in the thymus, and more specifically in the medullary thymic epithelial cells (mTECs), which also express a variety of other tissue-specific antigens. This thymic expression enables the maintenance of self-tolerance, and is essential in preventing auto-immune disease. Our laboratory has created a mouse mTEC clonal cell line specifically expressing insulin in order to better understand the regulatory mechanisms of this ectopic expression of insulin. In this study, we compared the insulin expressing cell line to an insulin non-expressing mTEC line by genome-wide expression profiling.ResultsThe most important difference was overexpression of CD34 in the insulin expressing clone, confirmed by Real-time Rt-PCR and flow cytometry. Cells in the thymus expressing higher levels of CD34 were found to contain higher levels of insulin and, to a lesser extent, Aire, a master regulator of self-antigen expression in the thymus. The cells expressing CD34 were not enriched in CD80, a known mTEC maturity marker.ConclusionCD34 may be a specific marker for functionality, with some specificity for insulin.