Prognostic Value of Complement Component 2 and Its Correlation with Immune Infiltrates in Hepatocellular Carcinoma
Prognostic Value of Complement Component 2 and Its Correlation with Immune Infiltrates in Hepatocellular Carcinoma
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补体成分2在肝细胞癌中的预后价值及其与免疫浸润的相关性
DOI：
10.1155/2020/3765937
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发表时间：
2020-06-15
影响因子：
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通讯作者：
Peng, Liang
中科院分区：
文献类型：
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作者：
Ning, Gang;Huang, Yan-Lin;Peng, Liang
背景补体成分2（C2）的单核苷酸多态性（SNP）与肝细胞癌（HCC）的发生密切相关。然而，关于C2在HCC中的作用和机制知之甚少。在本研究中，我们的目的是探讨C2的预后价值及其与肝癌中肿瘤浸润免疫细胞的相关性。材料与方法。从TCGA（365名HCC患者和50名健康对照）、GSE 14520（220名HCC患者和220名邻近正常组织）和ICGC HCC（232名HCC患者）队列下载mRNA表达。使用非配对Student'st检验或ANOVA检验来评估C2表达的差异。采用单因素和多因素分析C2的预后价值。CIBERSORT法计算22种肿瘤浸润免疫细胞的比例。与健康对照组相比，HCC中C2表达显著降低，且C2与TNM分期相关。多因素分析显示C2也是影响肝癌预后的独立因素。此外，在C2表达较高的HCC患者中发现了升高的CD4 T细胞，而在C2表达较低的HCC患者中发现了较高比例的巨噬细胞M0细胞。KEGG分析显示，C2高表达的HCC患者“细胞周期”、“AMPK信号通路”和“PPAR信号通路”富集。C2是HCC的预后因子，并可用作HCC未来治疗的治疗靶点。
Background. Single nucleotide polymorphism (SNP) of complement component 2 (C2) has been found to be significantly associated with hepatocellular carcinoma (HCC). However, little is known about the role and mechanism of C2 in HCC. In the present study, we aimed to explore the prognostic value of C2 and its correlation with tumor-infiltrating immune cells in HCC.Materials and Methods. mRNA expression was downloaded from TCGA (365 HCC patients and 50 healthy controls), GSE14520 (220 HCC patients and 220 adjacent normal tissues), and ICGC HCC (232 HCC patients) cohorts. Unpaired Student'st-tests or ANOVA tests were used to evaluate differences of C2 expression. Univariate and multivariate analyses were used to analyze the prognostic value of C2. CIBERSORT was used to calculate the proportion of 22 kinds of tumor-infiltrating immune cells.Results. Significantly lower C2 expression was found at HCC compared to healthy controls, and C2 was associated with TNM stages. Higher C2 expression was significantly associated with better prognosis, and multivariate analysis showed that C2 was also an independent factor for the prognosis of HCC. Moreover, elevated CD4 T cells were found at HCC patients with higher C2 expression while the higher proportion of macrophage M0 cells was found in HCC patients with lower C2 expression. KEGG analysis showed that "cell cycle," "AMPK signaling pathway," and "PPAR signaling pathway" were enriched in HCC patients with higher C2 expression.Conclusion. C2 is a prognostic factor for HCC and may be used as a therapeutic target for future treatment of HCC.