Activation of a dormant replication origin is essential for Haloferax mediterranei lacking the primary origins.
Activation of a dormant replication origin is essential for Haloferax mediterranei lacking the primary origins.
复制标题
休眠复制起点的激活对于缺乏原始起点的地中海盐鲆至关重要
DOI：
10.1038/ncomms9321
复制
发表时间：
2015-09-16
影响因子：
16.6
通讯作者：
Xiang H
中科院分区：
文献类型：
--
作者：
Yang H;Wu Z;Liu J;Liu X;Wang L;Cai S;Xiang H
在古细菌中已经证明了染色体复制的多个起点的使用。类似于真核生物中的休眠起源，古细菌中的一些潜在起源在基因组复制期间似乎是不活跃的。对地中海盐藻的资源利用进行了全面的探讨。在这里，我们报告了三个活跃的染色体起源的全基因组复制分析，并证明，当这三个起源被删除，一个休眠的起源被激活。值得注意的是，当其他起源已经不存在时，这个休眠起源不能被进一步删除，反之亦然。有趣的是，一个潜在的起源，似乎保持休眠在其原生宿主H。volcanii缺乏主要活性起点，当整合到起点缺失的H.医生。这些结果表明H.因此，如果需要的话，可以激活古细菌中休眠的复制起点。 在染色体复制过程中，有多个起始点，但在基因组复制过程中，一些潜在的起始点似乎是不活跃的。在这里，Yang等人表明，当从地中海盐藻中删除活性起源时，休眠起源被激活并且是必需的，这表明起源依赖性复制。
The use of multiple origins for chromosome replication has been demonstrated in archaea. Similar to the dormant origins in eukaryotes, some potential origins in archaea appear to be inactive during genome replication. We have comprehensively explored the origin utilization in Haloferax mediterranei. Here we report three active chromosomal origins by genome-wide replication profiling, and demonstrate that when these three origins are deleted, a dormant origin becomes activated. Notably, this dormant origin cannot be further deleted when the other origins are already absent and vice versa. Interestingly, a potential origin that appears to stay dormant in its native host H. volcanii lacking the main active origins becomes activated and competent for replication of the entire chromosome when integrated into the chromosome of origin-deleted H. mediterranei. These results indicate that origin-dependent replication is strictly required for H. mediterranei and that dormant replication origins in archaea can be activated if needed. Archaea use multiple origins for chromosome replication, but some potential origins appear to be inactive during the genome replication. Here, Yang et al. show that when active origins are deleted from Haloferax mediterranei, a dormant origin is activated and essential, suggesting an origin-dependent replication.