Evaluation of a RT-PCR based routine screening tool for the detection of disseminated epithelial cells in the bone marrow of breast cancer patients
Evaluation of a RT-PCR based routine screening tool for the detection of disseminated epithelial cells in the bone marrow of breast cancer patients
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DOI：
10.1007/s10549-008-0174-3
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发表时间：
2009-09-01
影响因子：
3.8
通讯作者：
Fehm, Tanja
中科院分区：
文献类型：
--
作者：
Becker, Sven;Becker-Pergola, Graziella;Fehm, Tanja
目的 大量研究表明，乳腺癌患者骨髓 (BM) 中存在临床隐匿性播散性肿瘤细胞 (DTC) 与不良的临床结果相关。免疫细胞化学 (ICC) 仍然是其检测的金标准。虽然基于 RT-PCR 的检测方法已经可用，但尚未用于 DTC 的常规检测。方法 为了评估检测的质量，我们使用标准化 ICC 和实时 RT-PCR 方案对 385 名患者的大型队列中的 DTC 检出率进行了直接比较。评估相关率，并将结果与临床数据进行比较。结果 ICC与RT-PCR之间存在显着相关性（P < 0.01）。阳性率相似（均为 35%），两种方法的结果在 73% 的病例中一致 (280/385)。结论 我们描述了一种基于实时 RT-PCR 的 DTC 检测方案，该方案专为常规临床实验室使用而设计。因此，RT-PCR 有潜力成为乳腺癌患者 BM 评估的替代检测方法。
Purpose Numerous studies have shown that the presence of clinically occult disseminated tumor cells (DTC's) in the bone marrow (BM) of breast cancer patients is associated with an unfavourable clinical outcome. Immunocytochemistry (ICC) remains the gold standard for their detection. While assays based on RT-PCR are available, they have not been used for routine detection of DTC's. Methods To assess the quality of the assay, we performed a direct comparison of DTC detection rates in a large cohort of 385 patients using both standardized ICC and real-time RT-PCR protocols. Correlation rates were assessed, and results were compared with clinical data. Results A significant correlation between ICC and RT-PCR was observed (P < 0.01). Positivity rates were similar (both 35%) and the results of both methods agreed in 73% of cases (280/385). Conclusions We describe a real-time RT-PCR based protocol for DTC-detection that has been specifically designed for routine clinical laboratory use. As such, RT-PCR has the potential to become an alternative testing method for BM evaluation in breast cancer patients.