Comparison of Lipid and Fatty Acid Composition of the Liver, Subcutaneous and Intra-abdominal Adipose Tissue, and Serum
Comparison of Lipid and Fatty Acid Composition of the Liver, Subcutaneous and Intra-abdominal Adipose Tissue, and Serum
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DOI：
10.1038/oby.2009.326
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发表时间：
2010-05-01
期刊：
影响因子：
6.9
通讯作者：
Oresic, Matej
中科院分区：
文献类型：
--
作者：
Kotronen, Anna;Seppanen-Laakso, Tuulikki;Oresic, Matej
神经酰胺可能介导饱和脂肪诱导的胰岛素抵抗，但没有数据比较人体组织之间的神经酰胺浓度。因此，我们对8名受试者的人皮下（SCfat）和腹腔内（IAfat）脂肪组织、肝脏和血清进行了脂质组学分析。与SCfat和IAfat相比，肝脏含有（nmol/mg组织）显著更多的神经酰胺（1.5-3倍）、鞘磷脂（7-8倍）、磷脂酰乙醇胺（10-11倍）、溶血磷脂酰胆碱（7-12倍），较少的醚连接磷脂酰胆碱（2-2.5倍），但二酰基甘油的量相似。神经酰胺及其合成前体（如棕榈酸（16：0）游离脂肪酸和鞘磷脂）的量在组织之间差异很大。肝脏中棕榈酸、硬脂酸（18：0）和长链多不饱和脂肪酸的比例高于脂肪组织。血清中反映的硬脂酰辅酶A去饱和酶1（SCD 1）活性（根据16：1/16：0的比例估计）与肝脏中的活性密切相关（r = 0.86，P = 0.024），但与脂肪组织无关。对于估计的延伸酶（18：1/16：1，r = 0.89，P = 0.01）、Delta 5（20：4/20：3，r = 0.89，P = 0.012）和Delta 6（18：3[n-6]/18：2，r = 1.0，P < 0.001）去饱和酶活性也是如此。我们的结论是，人的肝脏含有较高浓度的神经酰胺和饱和游离脂肪酸比SCfat或IAfat。
Ceramides may mediate saturated fat-induced insulin resistance, but there are no data comparing ceramide concentrations between human tissues. We therefore performed lipidomic analysis of human subcutaneous (SCfat) and intra-abdominal (IAfat) adipose tissue, the liver, and serum in eight subjects. The liver contained (nmol/mg tissue) significantly more ceramides (1.5-3-fold), sphingomyelins (7-8-fold), phosphatidylethanolamines (10-11-fold), lysophosphatidylcholines (7-12-fold), less ether-linked phosphatidylcholines (2-2.5-fold) but similar amounts of diacylglycerols as compared to SCfat and IAfat. The amounts of ceramides and their synthetic precursors, such as palmitic (16: 0) free fatty acids and sphingomyelins, differed considerably between the tissues. The liver contained proportionally more palmitic, stearic (18: 0), and long polyunsaturated fatty acids than adipose tissues. Stearoyl-CoA desaturase 1 (SCD1) activity reflected by serum, estimated from the 16:1/16:0-ratio, was closely related to that in the liver (r = 0.86, P = 0.024) but not adipose tissues. This was also true for estimated elongase (18:1/16:1, r = 0.89, P = 0.01), and Delta 5 (20:4/20:3, r = 0.89, P = 0.012) and Delta 6 (18:3[n-6]/18:2, r = 1.0, P < 0.001) desaturase activities. We conclude that the human liver contains higher concentrations of ceramides and saturated free fatty acids than either SCfat or IAfat.