Prevalence of gene expression additivity in genetically stable wheat allohexaploids
Prevalence of gene expression additivity in genetically stable wheat allohexaploids
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DOI：
10.1111/nph.12108
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发表时间：
2013-02-01
期刊：
影响因子：
9.4
通讯作者：
Chalhoub, Boulos
中科院分区：
文献类型：
--
作者：
Chelaifa, Houda;Chague, Veronique;Chalhoub, Boulos
基因表达的重编程是人工合成和天然异源多倍体的主要趋势，其中一个重要比例的基因表达被证明偏离亲本或亲本的平均值。高度稳定的合成小麦异源六倍体，其联合收割机了粗山羊草的D基因组和从天然六倍体提取的AB基因组小麦利用Affychip小麦基因组芯片对小麦全基因组范围内的转录变化进行了综合分析，在34820个表达的转录本中，99.3%的基因表达不偏离亲本的平均值，观察到基因表达加和性的普遍性。此外，当比较这些合成的和天然的小麦异源六倍体时，观察到几乎相似的表达（对于99.5%的基因）。这种近乎完全的加性从来没有报道过其他异源多倍体，更有趣的是，其他人工合成的小麦异源六倍体，不同于这里研究的具有天然四倍体Triticum turgidum作为AB基因组progenitors.Our的研究提供了见解的动态加性基因表达的高度稳定的小麦异源六倍体。
The reprogramming of gene expression appears as the major trend in synthetic and natural allopolyploids where expression of an important proportion of genes was shown to deviate from that of the parents or the average of the parents.In this study, we analyzed gene expression changes in previously reported, highly stable synthetic wheat allohexaploids that combine the D genome of Aegilops tauschii and the AB genome extracted from the natural hexaploid wheat Triticum aestivum. A comprehensive genome-wide analysis of transcriptional changes using the Affymetrix GeneChip Wheat Genome Array was conducted.Prevalence of gene expression additivity was observed where expression does not deviate from the average of the parents for 99.3% of 34 820 expressed transcripts. Moreover, nearly similar expression was observed (for 99.5% of genes) when comparing these synthetic and natural wheat allohexaploids. Such near-complete additivity has never been reported for other allopolyploids and, more interestingly, for other synthetic wheat allohexaploids that differ from the ones studied here by having the natural tetraploid Triticum turgidum as the AB genome progenitor.Our study gave insights into the dynamics of additive gene expression in the highly stable wheat allohexaploids.