Dissimilar kinetic behavior of electrically manipulated single- and double-stranded DNA tethered to a gold surface
Dissimilar kinetic behavior of electrically manipulated single- and double-stranded DNA tethered to a gold surface
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DOI：
10.1529/biophysj.105.078857
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发表时间：
2006-05-01
影响因子：
3.4
通讯作者：
Abstreiter, G
中科院分区：
文献类型：
--
作者：
Rant, U;Arinaga, K;Abstreiter, G
我们报告的电操纵的单链和双链的寡脱氧核苷酸，在电解质溶液中的黄金表面的末端拴系。交替排斥和吸引力的表面电场的响应进行了研究，通过时间分辨的荧光测量，揭示了显着不同的动态灵活的单链和刚性的双链DNA，分别。流体动力学模拟合理化这一发现，并公开了两种不同的动力学机制：刚性聚合物进行旋转周围的锚定枢轴点，柔性聚合物，另一方面，被拉到吸引表面段段。
We report on the electrical manipulation of single- and double-stranded oligodeoxynucleotides that are end tethered to gold surfaces in electrolyte solution. The response to alternating repulsive and attractive electric surface fields is studied by time-resolved fluorescence measurements, revealing markedly distinct dynamics for the flexible single-stranded and stiff double-stranded DNA, respectively. Hydrodynamic simulations rationalize this finding and disclose two different kinetic mechanisms: stiff polymers undergo rotation around the anchoring pivot point; flexible polymers, on the other hand, are pulled onto the attracting surface segment by segment.