An Abp1-dependent route of endocytosis functions when the classical endocytic pathway in yeast is inhibited.
An Abp1-dependent route of endocytosis functions when the classical endocytic pathway in yeast is inhibited.
复制标题
DOI：
10.1371/journal.pone.0103311
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Ayscough KR
中科院分区：
文献类型：
--
作者：
Aghamohammadzadeh S;Smaczynska-de Rooij II;Ayscough KR
网格蛋白介导的内吞作用（CME）是酵母和哺乳动物细胞中的一种充分表征的途径。越来越多的替代内吞途径，现在已被描述在哺乳动物细胞中，可以是网格蛋白，肌动蛋白，Arf 6依赖或独立。在酵母中，一个单一的网格蛋白介导的途径已被详细描述。然而，在许多突变株中这种途径的破坏表明可能存在其他摄取途径，至少对于大量脂质和液体内化。利用遗传学和活细胞成像的结合，我们在这里展示了S.酿酒酵母，不涉及先前显示与内吞作用的“经典”途径相关的几种蛋白质。这种替代途径在低水平的肌动蛋白破坏药物latrunculin-A的存在下发挥作用，该药物抑制蛋白Sla 1、Sla 2和Sac 6的运动，并且不依赖于发动蛋白功能。我们发现，在没有“经典”途径，肌动蛋白结合蛋白Abp 1现在是必不可少的散装内吞作用。这种新的途径似乎不同于另一种描述的S.这是因为它涉及至少一些已知与皮质肌动蛋白斑块相关的蛋白质，而不是在福尔马林依赖性内吞位点介导的蛋白质。这些数据表明，细胞有能力使用重叠组的组件，以促进在一系列条件下的内吞作用。
Clathrin-mediated endocytosis (CME) is a well characterized pathway in both yeast and mammalian cells. An increasing number of alternative endocytic pathways have now been described in mammalian cells that can be both clathrin, actin, and Arf6- dependent or independent. In yeast, a single clathrin-mediated pathway has been characterized in detail. However, disruption of this pathway in many mutant strains indicates that other uptake pathways might exist, at least for bulk lipid and fluid internalization. Using a combination of genetics and live cell imaging, here we show evidence for a novel endocytic pathway in S. cerevisiae that does not involve several of the proteins previously shown to be associated with the ‘classic’ pathway of endocytosis. This alternative pathway functions in the presence of low levels of the actin-disrupting drug latrunculin-A which inhibits movement of the proteins Sla1, Sla2, and Sac6, and is independent of dynamin function. We reveal that in the absence of the ‘classic’ pathway, the actin binding protein Abp1 is now essential for bulk endocytosis. This novel pathway appears to be distinct from another described alternative endocytic route in S. cerevisiae as it involves at least some proteins known to be associated with cortical actin patches rather than being mediated at formin-dependent endocytic sites. These data indicate that cells have the capacity to use overlapping sets of components to facilitate endocytosis under a range of conditions.
登录
查看更多内容
DOI：
10.1083/jcb.144.6.1203
发表时间：
1999-03-22
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cope MJ;Yang S;Shang C;Drubin DG
通讯作者：
Drubin DG
影响因子：
64.5
作者：
Kaksonen, M;Toret, CP;Drubin, DG
通讯作者：
Drubin, DG
影响因子：
3.3
作者：
Dewar, H;Warren, DT;Ayscough, KR
通讯作者：
Ayscough, KR
影响因子：
4
作者：
Gourlay, CW;Dewar, H;Ayscough, KR
通讯作者：
Ayscough, KR
影响因子：
3.3
作者：
Lila, T;Drubin, DG
通讯作者：
Drubin, DG