Electrical regulation of Schwann cells using conductive polypyrrole/chitosan polymers
Electrical regulation of Schwann cells using conductive polypyrrole/chitosan polymers
复制标题
使用导电聚吡咯/壳聚糖聚合物对雪旺细胞进行电调节
DOI：
10.1002/jbm.a.32511
复制
发表时间：
2010-04-01
影响因子：
4.9
通讯作者：
Luo, Zhuojing
中科院分区：
文献类型：
--
作者：
Huang, Jinghui;Hu, Xueyu;Luo, Zhuojing
在神经损伤的动物模型中，电刺激（ES）可以通过导电聚合物显著增强神经突起的生长，并加速周围神经的再生。因此，导电组织工程移植物与ES结合是神经损伤的潜在治疗方法。将雪旺细胞接种于导电支架上可获得导电组织工程支架。然而，当ES通过导电支架施加时，ES对雪旺细胞的影响从未被研究过。本研究以导电聚吡咯（PPy，2.5%）和可生物降解壳聚糖（97.5%）为原料，制备了一种可生物降解的导电复合材料，用于电刺激雪旺细胞。细胞凋亡实验检测雪旺细胞对ES的耐受性。使用DAPI染色和MTT测定表征细胞的生长。采用RT-PCR和Western blotting检测神经生长因子（NGF）和脑源性神经营养因子（BDNF）的mRNA和蛋白水平，采用ELISA法检测神经生长因子和脑源性神经营养因子的分泌量。结果表明，PPy/壳聚糖膜支持细胞粘附，铺展，和增殖与ES或没有。有趣的是，FS通过PPy/壳聚糖复合物应用显着增强的表达和分泌的NGF和BDNF相比，没有ES的对照细胞。这些发现首次强调了通过ES增加神经营养因子分泌来增强导电支架中神经再生的可能性。9 2009年威利期刊公司。J Biomed Mater Res 93A：164-174，2010
Electrical stimulation (ES) can dramatically enhance neurite outgrowth through conductive polymers and accelerate peripheral nerve regeneration in animal models of nerve injury. Therefore, conductive tissue engineering graft in combination with ES is a potential treatment for neural injuries. Conductive tissue engineering graft can be obtained by seeding Schwann cells on conductive scaffold. However, when ES is applied through the conductive scaffold, the impact of ES on Schwann cells has never been investigated. In this study, a biodegradable conductive composite made Of conductive polypyrrole (PPy, 2.5%) and biodegradable chitosan (97.5%,) was prepared in order to electrically Stimulate Schwann cells. The tolerance of Schwann cells to ES was examined by a cell apoptosis assay. The growth of the cells was characterized using DAPI staining and a MTT assay. mRNA and protein levels of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) in Schwann cells were assayed by RT-PCR and Western blotting, and the amount of NGF and BDNF secreted was determined by an ELISA assay. The results showed that the PPy/chitosan membranes supported cell adhesion, spreading, and proliferation with or without ES. Interestingly, FS applied through the PPy/chitosan composite dramatically enhanced the expression and secretion of NGF and BDNF when compared with control cells without ES. These findings highlight for the first time the possibility of enhancing nerve regeneration in conductive scaffolds through ES-increased neurotrophin secretion. 9 2009 Wiley Periodicals, Inc. J Biomed Mater Res 93A: 164-174, 2010