TENSILE-STRENGTH OF CELL-WALLS OF LIVING CELLS

TENSILE-STRENGTH OF CELL-WALLS OF LIVING CELLS
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DOI:
10.1104/pp.79.2.485
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发表时间:
1985-01-01
期刊:
影响因子:
7.4
通讯作者:
CARPITA, NC
CARPITA, NC
中科院分区:
生物学1区
文献类型:
--
作者:
CARPITA, NC

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采用气体减压技术测定了单细胞细胞壁的断裂强度。鼠伤寒沙门菌和单细胞绿藻衣藻的破壁强度分别为100和95个大气压,水霉菌孢子体的破壁强度为65个大气压,胡萝卜悬浮培养细胞的破壁强度仅为30个大气压。基于破裂压力、细胞半径和壁厚的壁拉应力估计表明,微纤维壁不一定比原始生物的壁强。因此,必须考虑它们进化的其他假设。
A gas decompression technique was used to determine the breaking strength of cell walls of single cells. Breaking strengths of the bacterium Salmonella typhimurium and the unicellular green alga Chlamydomonas eugametos were 100 and 95 atmospheres, respectively, while those of sporophytes of the water mold Blastocladiella emersonii were 65 atmospheres, and those of suspension cultured cells of carrot were only 30 atmospheres. Estimation of wall tensile stress based on breaking pressures, cell radii, and estimation of wall thickness, indicates that microfibrillar walls are not necessarily stronger than walls of primitive organisms. Hence, alternative hypotheses for their evolution must be considered.