Kinetic Study of Interaction between Solute Molecule and Surfactant Micelle
Kinetic Study of Interaction between Solute Molecule and Surfactant Micelle
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溶质分子与表面活性剂胶束相互作用的动力学研究
DOI：
10.2116/analsci.31.1019
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发表时间：
2015
影响因子：
1.6
通讯作者：
Youki Shimazaki
中科院分区：
文献类型：
--
作者：
K. Miyabe;R. Takahashi;Youki Shimazaki
用毛细管电泳色谱(MKCCE)方法建立了亲和动力学矩分析方法，用于分子间相互作用的动力学研究。用MKCCE法测定了百里香酚与十二烷基硫酸钠胶束在胶束电动色谱(MEKC)体系中相互作用的缔合和解离速率常数。它是一种基于矩理论的研究分子间相互作用动力学的方法，它不需要对分子进行固定和化学修饰。在CCE模式下，控制实验条件，阻止溶质-胶束复合体的迁移，只有溶质分子在毛细管中迁移。溶质分子在CCE体系中的传质行为类似于在色谱体系中的传质行为。然而，由于实际中很难在CCE条件下真正进行CE实验，所以在溶质-胶束络合物的迁移速度为零的条件下，随着实验条件的变化，即外加压力的变化，CE数据被测量。可以从CE数据中解析地确定速率常数。在MKCCE方法中，为了确定速率常数，不需要将数值计算的洗脱曲线与实验测量的洗脱曲线进行拟合。百里香酚与十二烷基硫酸钠胶束相互作用的缔合平衡常数和缔合与解离速率常数分别为6.35×10^3dm^1、5.6×10^4dm^3m ol·−1、5.6×10^4dm^1·S·−1和8.7 S·−·1。结果表明，MKCCE方法是研究分子间相互作用动力学的有效方法。
We developed moment analysis of affinity kinetics by chromatographic capillary electrophoresis (MKCCE) method for the kinetic study of intermolecular interactions. Association and dissociation rate constants of the interaction in a micellar electrokinetic chromatography (MEKC) system between thymol and sodium dodecylsulfate micelle were determined by the MKCCE method. It is a method based on the moment theory for the kinetic study of intermolecular interactions under the conditions that neither immobilization nor chemical modification of molecules is required. In CCE mode, experimental conditions are controlled so that the migration of solute-micelle complex is stopped and only solute molecules migrate in a capillary. Mass transfer behavior of solute molecules in the CCE system is analogous to that in a chromatographic system. However, because it was difficult in practice to really perform CE experiments under the CCE conditions, CE data were measured with changing experimental conditions, i.e. , applied pressure, under the conditions that the migration velocity of solute-micelle complex was around zero. The rate constants could be analytically determined from the CE data. In the MKCCE method, it is not necessary to fit elution curves numerically calculated to those experimentally measured for the determination of the rate constants. Regarding the interaction between thymol and SDS micelles, association equilibrium constant and association and dissociation rate constants were determined as 6.35 × 10^3 dm^3 mol^−1, 5.6 × 10^4 dm^3 mol^−1 s^−1, and 8.7 s^−1, respectively. It was demonstrated that the MKCCE method was effective for the kinetic study of intermolecular interactions.