SARS-CoV-2 RNA in Wastewater Settled Solids Is Associated with COVID-19 Cases in a Large Urban Sewershed
SARS-CoV-2 RNA in Wastewater Settled Solids Is Associated with COVID-19 Cases in a Large Urban Sewershed
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DOI：
10.1021/acs.est.0c06191
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发表时间：
2021-01-05
影响因子：
11.4
通讯作者：
Boehm, Alexandria B.
中科院分区：
文献类型：
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作者：
Graham, Katherine E.;Loeb, Stephanie K.;Boehm, Alexandria B.
基于废水的流行病学可能有助于为公共卫生应对由SARS-CoV-2引起的新冠肺炎等病毒性疾病提供信息。我们对两个污水处理厂的废水进水和初级沉淀固体中的SARS-CoV-2RNA进行了量化，以提供分析前和分析方法，并评估进水和固体中是否含有更多的病毒靶标。初步沉淀的固体样品的SARS-CoV-2的检出频率高于相应的进境样品。同样，使用一步数字滴状(Dd)RT-PCR比两步RT-QPCR和两步ddRT-PCR更容易在固体中检测到SARS-CoV-2RNA，这可能是因为一步ddRT-PCR方法减少了抑制。随后，我们分析了来自单一工厂的89个沉淀固体样本的纵向时间序列，以检测SARS-CoV-2RNA以及冠状病毒恢复(牛冠状病毒)和粪便强度(辣椒轻度斑驳病毒)的控制。SARS-CoV-2RNA指标N1和N2浓度与污水处理厂新冠肺炎临床确诊病例数呈显著正相关。综上所述，结果表明，检测沉淀物中的SARS-CoV-2RNA浓度可能比检测进水中的SARS-CoV-2RNA更敏感。
Wastewater-based epidemiology may be useful for informing public health response to viral diseases like COVID-19 caused by SARS-CoV-2. We quantified SARS-CoV-2 RNA in wastewater influent and primary settled solids in two wastewater treatment plants to inform the preanalytical and analytical approaches and to assess whether influent or solids harbored more viral targets. The primary settled solids samples resulted in higher SARS-CoV-2 detection frequencies than the corresponding influent samples. Likewise, SARS-CoV-2 RNA was more readily detected in solids using one-step digital droplet (dd)RT-PCR than with two-step RT-QPCR and two-step ddRT-PCR, likely owing to reduced inhibition with the one-step ddRT-PCR assay. We subsequently analyzed a longitudinal time series of 89 settled solids samples from a single plant for SARS-CoV-2 RNA as well as coronavirus recovery (bovine coronavirus) and fecal strength (pepper mild mottle virus) controls. SARS-CoV-2 RNA targets N1 and N2 concentrations correlated positively and significantly with COVID-19 clinically confirmed case counts in the sewershed. Together, the results demonstrate that measuring SARS-CoV-2 RNA concentrations in settled solids may be a more sensitive approach than measuring SARS-CoV-2 in influent.