Activation of flavin-containing oxidases underlies light-induced production of H2O2 in mammalian cells
Activation of flavin-containing oxidases underlies light-induced production of H2O2 in mammalian cells
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DOI：
10.1073/pnas.96.11.6255
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发表时间：
1999-05-25
影响因子：
11.1
通讯作者：
White, JG
中科院分区：
文献类型：
--
作者：
Hockberger, PE;Skimina, TA;White, JG
紫蓝色光对哺乳动物细胞有毒性，这种毒性与细胞产生过氧化氢有关。在这份报告中，我们表明，紫蓝色光和UVA一样，可以刺激培养的小鼠、猴子和人类细胞产生过氧化氢。T-VE发现过氧化氢起源于过氧化物体和线粒体，并且在含有黄素的细胞中过表达的氧化物酶增强。这些结果支持这样的假设，即黄素蛋白的光还原是光诱导细胞产生过氧化氢的基础。由于过氧化氢及其代谢物--羟基自由基会导致细胞损伤，这些活性氧物种可能会导致与紫外线、紫外光和蓝光相关的病理变化。它们还可能导致在光学显微镜下经常遇到的光毒性。由于1,047 nm波长的多光子激发成像可能通过最小化黄素蛋白的光还原来阻止光诱导细胞中过氧化氢的产生，因此这项技术可能有助于降低荧光显微镜中的光毒性。
Violet-blue light is toxic to mammalian cells, and this toxicity has been linked with cellular production of H2O2. In this report, we show, that violet-blue light, as well as UVA, stimulated H2O2 production in cultured mouse, monkey, and human cells. T-Ve found that H2O2 originated in peroxisomes and mitochondria, and it was enhanced in cells overexpressing flavin containing oxidases. These results support the hypothesis that photoreduction of flavoproteins underlies light-induced production of H2O2 in cells. Because H2O2 and its metabolite, hydroxyl radicals, can cause cellular damage, these reactive oxygen species may contribute to pathologies associated with exposure to UVA, violet, and blue light. They may also contribute to phototoxicity often encountered during light microscopy. Because multiphoton excitation imaging with 1,047-nm wavelength prevented light-induced H2O2 production in cells, possibly by minimizing photoreduction of flavoproteins, this technique may be useful for decreasing phototoxicity during fluorescence microscopy.