Involvement of vesicular trafficking system in membrane targeting of the progeny influenza virus genome
Involvement of vesicular trafficking system in membrane targeting of the progeny influenza virus genome
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DOI：
10.1016/j.micinf.2010.06.011
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发表时间：
2010-11-01
影响因子：
5.8
通讯作者：
Nagata, Kyosuke
中科院分区：
文献类型：
--
作者：
Jo, Shuichi;Kawaguchi, Atsushi;Nagata, Kyosuke
A型流感病毒的基因组由负极性的单链RNA组成，在细胞核中复制的子代病毒RNA（vRNA）是核输出的，最后被运输到质膜下的出芽位点，然而，尽管病毒蛋白和细胞骨架被认为起作用，但vRNA的膜靶向的精确过程尚不清楚。荧光原位杂交方法我们的结果提供了一个证据，证明囊泡运输参与了子代vRNA的膜靶向，而不依赖于病毒膜蛋白的靶向（C）2010巴斯德研究所出版Elsevier Masson SAS版权所有
The genome of influenza type A virus consists of single-stranded RNAs of negative polarity Progeny viral RNA (vRNA) replicated in the nucleus is nuclear-exported, and finally transported to the budding site beneath the plasma membrane However, the precise process of the membrane targeting of vRNA is unclear although viral proteins and cytoskeleton are thought to play roles Here we have visualized the translocation process of progeny vRNA using fluorescence in situ hybridization method Our results provide an evidence of the Involvement of vesicular trafficking in membrane targeting of progeny vRNA independent of that of viral membrane proteins (C) 2010 Institut Pasteur Published by Elsevier Masson SAS All rights reserved