Molecular detection of fungi carried by Bradysia difformis (Sciaridae: Diptera) in South African forestry nurseries
Molecular detection of fungi carried by Bradysia difformis (Sciaridae: Diptera) in South African forestry nurseries
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DOI：
10.2989/shfj.2007.69.2.5.291
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发表时间：
2007-10-01
期刊：
影响因子：
--
通讯作者：
Wingfield, M. J.
中科院分区：
文献类型：
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作者：
Hurley, B. P.;Slippers, B.;Wingfield, M. J.
异型迟眼蕈蚊（Sciaridae：双翅目）最近从南非林业苗圃中发现，并被认为已被引入该国。真菌蚊类，包括徐长卿属，传播各种真菌病原体。因此假设B.异形镰刀菌可能是负责在南非林业苗圃内的病原体镰刀菌的快速蔓延。然而，以前的研究未能证实这一假设。在这项研究中，我们试图确定B。difformis和两个苗圃病原体F circinatum和灰葡萄孢，使用敏感的DNA为基础的标记。从南非的四个主要林业苗圃中获得了总共60个真菌蚊和四个25-30个真菌蚊的组合集合。利用种特异性引物CIRC 1A和CIRC 4A以及C729+和C729-对卷叶镰刀菌和BO进行了检测。cinerea，分别。当真菌DNA与真菌蚊DNA混合时，在不同浓度下测试这些引物的灵敏度。用通用真菌引物检测B上的任何其他真菌。畸形无论是F circinatum，波。B上未检测到灰霉病菌或任何其他真菌病原体。畸形尽管CIRC 1A和CIRC 4A在最低13.4pg和1：3727真菌与真菌蚊DNA的比例下检测卷叶镰刀菌的能力，以及C729+和C729-检测Bo的能力。灰霉病菌DNA含量最低为13.4pg，真菌与真菌蚊DNA的比例为1：14691。用真菌通用引物检测到其他真菌，但这些真菌都不是病原菌。我们得出结论，B.在南非森林苗圃中，异形菌在这些或其他真菌病原体的运动中不起主要作用。
Bradysia difformis (Sciaridae: Diptera) has recently been identified from South African forestry nurseries, and is thought to have been introduced into the country. Fungus gnats, including Bradysia spp., are known to transmit various fungal pathogens. It has thus been hypothesised that B. difformis might be responsible for the rapid spread of the pathogen Fusarium circinatum within South African forestry nurseries. Previous studies have, however, failed to confirm this assumption. In this study we attempted to determine the association between B. difformis and the two nursery pathogens F circinatum and Botrytis cinerea, using sensitive DNA-based markers. A total of 60 fungus gnats and four combined collections of 25-30 fungus gnats were obtained from four of the major forestry nurseries in South Africa. The species-specific primers CIRC1A and CIRC4A and C729+ and C729- were used in an attempt to detect F circinatum and Bo. cinerea, respectively. The sensitivity of these primers when fungal DNA was mixed with fungus gnat DNA was tested at various concentrations. General fungal primers were used to detect any other fungi on B. difformis. Neither F circinatum, Bo. cinerea nor any other fungal pathogens were detected on B. difformis. This is despite the ability of CIRC1A and CIRC4A to detect F circinatum at a minimum of 13.4pg and a ratio of 1:3 727 fungus to fungus gnat DNA, and the ability of C729+ and C729- to detect Bo. cinerea at a minimum of 13.4pg and a ratio of 1:14 691 fungus to fungus gnat DNA. Other fungi were detected using the general fungal primers, but none of these fungi were pathogens. We conclude that B. difformis does not play a major role in the movement of these or other fungal pathogens in South African forestry nurseries.