THE ATPASE ACTIVITY OF RECA IS NEEDED TO PUSH THE DNA STRAND EXCHANGE THROUGH HETEROLOGOUS REGIONS
THE ATPASE ACTIVITY OF RECA IS NEEDED TO PUSH THE DNA STRAND EXCHANGE THROUGH HETEROLOGOUS REGIONS
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DOI：
10.1002/j.1460-2075.1991.tb05017.x
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发表时间：
1991-12-01
期刊：
影响因子：
11.4
通讯作者：
STASIAK, A
中科院分区：
文献类型：
--
作者：
ROSSELLI, W;STASIAK, A
在RecA介导的重组反应中ATP水解的作用在本文中得到解决。最近的研究表明，RecA促进的完全同源的双链和单链DNA之间的DNA链交换可以在不存在ATP水解的情况下非常有效。在这项工作中，我们证明了来自ATP水解的能量是严格需要驱动DNA链交换通过相互作用的DNA分子不在同源寄存器的区域。因此，如前所述，除了ATP水解在促进RecA从重组反应产物中解离的作用外，ATP水解在链交换的实际过程中也起着关键作用，前提是同源配准的缺乏阻碍了分支迁移的过程。
The role of ATP hydrolysis during the RecA-mediated recombination reaction is addressed in this paper. Recent studies indicated that the RecA-promoted DNA strand exchange between completely homologous double- and single-stranded DNA can be very efficient in the absence of ATP hydrolysis. In this work we demonstrate that the energy derived from the ATP hydrolysis is strictly needed to drive the DNA strand exchange through the regions where the interacting DNA molecules are not in a homologous register. Therefore, in addition to the role of the ATP hydrolysis in promoting the dissociation of RecA from the products of the recombination reaction, as described earlier, ATP hydrolysis also plays a crucial role in the actual process of strand exchange, provided that the lack of homologous register obstructs the process of branch migration.