Antioxidant Activities of the Supercritical and Conventional Satureja montana Extracts
Antioxidant Activities of the Supercritical and Conventional Satureja montana Extracts
复制标题
DOI：
10.1111/j.1750-3841.2009.01376.x
复制
发表时间：
2009-11-01
影响因子：
3.9
通讯作者：
Palavra, A. M. F.
中科院分区：
文献类型：
--
作者：
Grosso, C.;Oliveira, A. C.;Palavra, A. M. F.
比较了超临界流体萃取法（SFE）、水蒸气蒸馏法（HD）和索氏提取法（SE）提取的温州蜜柑挥发性和非挥发性组分的抗氧化活性。DPPH法和Rancimat法之间的一致性良好，表明该提取物能够清除自由基，抑制脂质氧化。挥发油（在90 bar/40 ℃下通过SFE获得）是最有效的提取物，表现出最低的EC（50）（0.06 g/L）和最高的保护因子（PF = 2.03）。这些结果表明，超临界流体萃取技术的优势，通过避免热降解和水解反应的传统技术。此外，挥发油中的百里醌含量是精油（HD）的15倍。这种化合物由于其抗氧化、神经保护和抗癌活性而非常重要。香芹酚+麝香草酚+麝香草醌在挥发油中的组合可能是负责的抗氧化活性的增加相比，HD，这表明，在这种情况下，超临界流体萃取提高了最终产品的价值。
The antioxidant activities of the volatile and the nonvolatile fractions from Satureja montana obtained by supercritical fluid extraction (SFE) and by conventional techniques, hydrodistillation (HD) and soxhlet extraction (SE), were compared. A good agreement between DPPH and rancimat methods was obtained showing that the extracts were able to scavenge free radicals and to inhibit lipid oxidation. The volatile oil (obtained by SFE at 90 bar/40 degrees C) was the most effective extract, presenting the lowest EC(50) (0.06 g/L) and the highest protector factor (PF = 2.03). These results demonstrated the advantages of SFE over conventional techniques by avoiding thermodegradation and hydrolysis reactions. Furthermore, volatile oil is 15 times richer in thymoquinone than the essential oil (HD). This compound is of great importance due to its antioxidant, neuroprotective, and anti-cancer activities. The combination of carvacrol + thymol + thymoquinone in volatile oil may be responsible for the increase in the antioxidant activity when compared to HD, which demonstrates that, in this case, SFE improves value to the final product.