Intragenic tandem repeat variation between Legionella pneumophila strains.
Intragenic tandem repeat variation between Legionella pneumophila strains.
复制标题
DOI：
10.1186/1471-2180-8-218
复制
发表时间：
2008-12-10
期刊：
影响因子：
4.2
通讯作者：
Anné J
中科院分区：
文献类型：
--
作者：
Coil DA;Vandersmissen L;Ginevra C;Jarraud S;Lammertyn E;Anné J
细菌基因组中含有大量的串联重复序列，然而，在基因编码区内发现的这些重复序列可能产生的表型效应才刚刚开始被研究。来自其他生物体的证据表明，这些重复序列可能参与新基因的进化、基因调节、适应、对环境压力的抵抗和对免疫系统的回避。在这项研究中，我们调查了嗜肺军团菌基因组中基因内串联重复序列拷贝数的存在和变异性，嗜肺军团菌是一种被称为军团病的严重肺炎的病原体。在费城菌株的基因组中，我们使用保守选择标准鉴定了26个基因内串联重复序列。其中，7个在大量嗜肺乳杆菌血清1组菌株之间的重复拷贝数是“多态性”的。这些菌株是从多个地理区域的各种环境和患者中收集的。在这组菌株中，除了一个基因外，这7个基因在不同来源（环境、临床和温泉）的样本中显示出统计学上不同的重复拷贝数模式。这些结果支持了基因内串联重复序列可能在毒力和对不同环境的适应中发挥作用的假设。虽然串联重复序列是包括嗜肺乳杆菌在内的原核生物分子分型研究中越来越受欢迎的焦点，但这项研究是首次研究串联重复序列分布的差异作为临床或环境起源的功能。
Bacterial genomes harbour a large number of tandem repeats, yet the possible phenotypic effects of those found within the coding region of genes are only beginning to be examined. Evidence exists from other organisms that these repeats can be involved in the evolution of new genes, gene regulation, adaptation, resistance to environmental stresses, and avoidance of the immune system. In this study, we have investigated the presence and variability in copy number of intragenic tandemly repeated sequences in the genome of Legionella pneumophila, the etiological agent of a severe pneumonia known as Legionnaires' disease. Within the genome of the Philadelphia strain, we have identified 26 intragenic tandem repeat sequences using conservative selection criteria. Of these, seven were "polymorphic" in terms of repeat copy number between a large number of L. pneumophila serogroup 1 strains. These strains were collected from a wide variety of environments and patients in several geographical regions. Within this panel of strains, all but one of these seven genes exhibited statistically different patterns in repeat copy number between samples from different origins (environmental, clinical, and hot springs). These results support the hypothesis that intragenic tandem repeats could play a role in virulence and adaptation to different environments. While tandem repeats are an increasingly popular focus of molecular typing studies in prokaryotes, including in L. pneumophila, this study is the first examining the difference in tandem repeat distribution as a function of clinical or environmental origin.
登录
查看更多内容
影响因子：
15.9
作者：
Bayliss, CD;Field, D;Moxon, ER
通讯作者：
Moxon, ER
影响因子：
3.4
作者：
Chen, John;Reyes, Moraima;Shuman, Howard A.
通讯作者：
Shuman, Howard A.
影响因子：
--
作者：
Borella, Paola;Marchesi, Isabella;Marchegiano, Patrizia
通讯作者：
Marchegiano, Patrizia
影响因子：
4.2
作者：
MARRAO, G;VERISSIMO, A;DACOSTA, MS
通讯作者：
DACOSTA, MS
影响因子：
14.9
作者：
Benson, G
通讯作者：
Benson, G