Molecular identification and characterization of Elsinoe murrayae (Synonym: Sphaceloma murrayae) from weeping willow
Molecular identification and characterization of Elsinoe murrayae (Synonym: Sphaceloma murrayae) from weeping willow
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垂柳Elsinoe murrayae（别名：Sphaceloma murrayae）的分子鉴定和表征
DOI：
10.1111/jph.12670
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发表时间：
2018
影响因子：
1.5
通讯作者：
Cheng Qiang
中科院分区：
文献类型：
--
作者：
Zhao Lijuan;Zhang Weitao;Xiao Hongju;Xiong Qin;Cheng Qiang
Sphaceloma murrayaeis a significant fungal pathogen ofSalixspp. It causes greyish‐white leaf spots, which were reported worldwide except in China. Its morphological characteristics were described in the early literature; however, there is a lack of molecular information pertaining to this fungus. This study identified and characterized three fungal isolates that obtained from weeping willow leaf spots in China. Based on disease symptoms, morphological characteristics and single nomenclature rules for fungi, these isolates are proposed to be new combinations ofElsinoë murrayae(Synonym:S. murrayae). Phylogenetic analysis that combined internal transcribed spacer (ITS), large subunit (LSU),RBP2andTEF1‐α DNA sequences indicated thatE. murrayaeisolates andElsinoë salicina—anotherElsinoësp. isolated fromSalixsp.—were distinguishable species. With trypan blue staining and stereomicroscopic observation, we found that large‐scale cell death occurred at 2 days postinoculation (dpi) and slight disease symptoms started at 3 dpi when the conidia were inoculated onSalix babylonicaleaves. Pathogenicity analysis revealed that three isolates can successfully infect mature leaves ofS. babylonica,Salix fragilisandSalix suchowensis,but notSalix matsudana. In addition, a necrosis‐ and ethylene‐inducing‐like proteins’ (NLP) gene, namedEmNLP1, was cloned. The cytotoxicity of EmNLP1 was confirmed by transient assay in tobacco. During infection,EmNLP1dramatically peaked at 2 dpi and maintained a high‐level expression in the necrosis lesion growing stage.
