Manganese-doped ZnSe quantum dots as a probe for time-resolved fluorescence detection of 5-fluorouracil.
Manganese-doped ZnSe quantum dots as a probe for time-resolved fluorescence detection of 5-fluorouracil.
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DOI：
10.1021/ac202101u
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发表时间：
2011-11
影响因子：
7.4
通讯作者：
Dongmei Zhu;Yun‐Zi Chen;Liping Jiang;Jun‐shan Geng;Jianrong Zhang;Jun‐Jie Zhu
中科院分区：
文献类型：
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作者：
Dongmei Zhu;Yun‐Zi Chen;Liping Jiang;Jun‐shan Geng;Jianrong Zhang;Jun‐Jie Zhu
量子点（QD）通常用于常规荧光检测。然而，由于量子点发光寿命短，难以应用于时间分辨荧光分析。本文采用一种绿色的微波辅助水溶液快速合成方法制备了具有长寿命发光的高质量Mn掺杂ZnSe量子点。Mn掺杂的ZnSe量子点的荧光寿命延长了400 μs，是CdS、CdSe和CdTe等传统量子点的1万倍。在260 nm连续光照下，量子点的光稳定性超过35 h。用巯基丙酸（MPA）封端的Mn掺杂的ZnSe量子点用于5-氟尿嘧啶（5-FU）的时间分辨荧光检测，检测限为128 nM。1.5 μM 5-FU的7次独立测定的相对标准偏差为3.8%，回收率范围为93%-106%。结果表明，Mn掺杂的ZnSe量子点可以作为一个很好的候选人作为高灵敏度的时间分辨荧光分析的发光探针。
Quantum dots (QDs) are generally used for the conventional fluorescence detection. However, it is difficult for the QDs to be applied in time-resolved fluorometry due to their short-lived emission. In this paper, high-quality Mn-doped ZnSe QDs with long-lived emission were prepared using a green and rapid microwave-assisted synthetic approach in aqueous solution. Fluorescence lifetime of the Mn-doped ZnSe QDs was extended as long as 400 μs, which was 10,000 times higher than that of conventional QDs such as CdS, CdSe, and CdTe. The QDs exhibited an excellent photostability over 35 h under continuous irradiation at 260 nm. Capped with mercaptopropionic acid (MPA), the Mn-doped ZnSe QDs were used for the time-resolved fluorescence detection of 5-fluorouracil (5-FU) with the detection limit of 128 nM. The relative standard deviation for seven independent measurements of 1.5 μM 5-FU was 3.8%, and the recovery ranged from 93% to 106%. The results revealed that the Mn-doped ZnSe QDs could be a good candidate as a luminescence probe for highly sensitive time-resolved fluorometry.