The effect of lactoferrin on oral bacterial attachment
The effect of lactoferrin on oral bacterial attachment
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DOI：
10.1111/j.1399-302x.2009.00537.x
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发表时间：
2009-10-01
影响因子：
--
通讯作者：
Wu, C. D.
中科院分区：
文献类型：
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作者：
Arslan, S. Y.;Leung, K. P.;Wu, C. D.
前言：乳铁蛋白（Lf）是一种与铁结合的唾液糖蛋白，在人类抵抗局部粘膜感染的先天性防御中起重要作用。我们假设Lf通过铁螯合作用干扰初始口腔细菌附着于表面，从而抑制随后的生物膜形成。目的是探讨Lf对单菌种和多菌种口腔生物膜形成早期阶段的影响。方法：本研究使用戈登链球菌、变形链球菌、具核梭杆菌和牙龈卟啉单胞菌。将涂有流动的人工唾液（0.3ml/min）的双轨道流动池的玻璃盘用于研究细菌附着（3 h，37 ℃），所述流动的人工唾液具有和不具有Lf（100 μ g/ml）。还通过用LF包被的（20-100 μ g/ml）和未包被的载玻片孵育单一或多种试验细菌（10（7）菌落形成单位/ml）来检查附着。还检测了β-乳球蛋白、2，2 ′-联吡啶（25-100 μ g/ml）、铁螯合剂和FeCl 3对S. gordonii（50.3%，P < 0.05），而F.具核菌和牙龈卟啉单胞菌。然而，含S. gordonii（即S. gordonii/F. nucleatum或S. gordonii/P. gingivalis）分别降低48.7%和62.1%（P < 0.05）。β-Lactoglobulin对S. gordonii。在100 μ m2，2 ′-联吡啶存在下，S.戈登氏菌减少了53.87%。在S中没有观察到附着减少。结论：Lf可抑制S. gordonii的初始粘附。gordonii和S.戈登氏菌通过铁螯合作用共聚集。这可能导致随后抑制口腔生物膜的形成。
Introduction:Lactoferrin (Lf), an iron-binding salivary glycoprotein, plays an important role in human innate defense against local mucosal infection. We hypothesized that Lf interferes with initial oral bacterial attachment to surfaces by iron sequestration, so inhibiting subsequent biofilm formation. The objective was to investigate the effect of Lf on the early stages of single-species and multi-species oral biofilm development.Methods:Streptococcus gordonii, Streptococcus mutans, Fusobacterium nucleatum and Porphyromonas gingivalis were used in this study. Glass disks of a two-track flow cell coated with flowing artificial saliva (0.3 ml/min) with and without Lf (100 mu g/ml) were used for studying bacterial attachment (3 h, 37 degrees C). Attachment was also examined by incubating single or multiple species of test bacteria (10(7) colony-forming units/ml) with Lf-coated (20-100 mu g/ml) and uncoated glass slides. The effects of beta-lactoglobulin, 2,2'-dipyridyl (25-100 mu g/ml), an iron chelator, and FeCl3 on attachment were also examined.Results:Lf inhibited the initial attachment of S. gordonii (50.3%, P < 0.05) but not that of F. nucleatum and P. gingivalis. However, the attachment of a dual-species biofilm containing S. gordonii (i.e. S. gordonii/F. nucleatum or S. gordonii/P. gingivalis) was significantly reduced (48.7% or 62.1%, respectively, P < 0.05) in the presence of Lf. beta-Lactoglobulin did not affect the attachment of S. gordonii. In the presence of 100 mu m 2,2'-dipyridyl, attachment of S. gordonii was reduced by 53.87%. No reduction in attachment was noted in S. gordonii pretreated with Lf (100 mu g/ml) and FeCl3 (20-200 mu m).Conclusion:Lf suppresses initial attachment of S. gordonii and S. gordonii coaggregates by iron sequestration. This may lead to subsequent inhibition of oral biofilm development.