Isolations of Cache Valley Virus From Aedes albopictus (Diptera: Culicidae) in New Jersey and Evaluation of Its Role as a Regional Arbovirus Vector
Isolations of Cache Valley Virus From Aedes albopictus (Diptera: Culicidae) in New Jersey and Evaluation of Its Role as a Regional Arbovirus Vector
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DOI：
10.1603/me13099
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发表时间：
2013-11-01
影响因子：
2.1
通讯作者：
Andreadis, Theodore G.
中科院分区：
文献类型：
--
作者：
Armstrong, Philip M.;Anderson, John F.;Andreadis, Theodore G.
亚洲虎蚊，白纹伊蚊（Skuse），是一种入侵物种，是新泽西城市和郊区的主要害虫问题。为了评估其作为虫媒病毒载体的潜在作用，我们采样了Ae。白纹伊蚊从两个新泽西县超过3年的时间，并估计病毒感染的患病率Vero细胞培养和逆转录聚合酶链反应测定。从34，567份野外采集的Ae.经分子生物学鉴定，均为Cache Valley病毒。AE. 2011年7月底至9月初在美世县采集的白纹伊蚊（N = 3，138）也通过逆转录-聚合酶链反应进行了西尼罗河病毒（WNV）复检，结果均为阴性。这些结果证实了以前的研究结果表明，Ae。在自然界中，白纹伊蚊偶尔会感染鹿相关虫媒病毒Cache Valley病毒。与此相反，我们没有检测到西尼罗河病毒感染的Ae。白纹伊蚊，尽管同时在该地区的西尼罗河病毒扩增。
The Asian tiger mosquito, Aedes albopictus (Skuse), is an invasive species and a major pest problem in urban and suburban locales in New Jersey. To assess its potential role as an arbovirus vector, we sampled Ae. albopictus from two New Jersey counties over a 3-yr period and estimated the prevalence of virus infection by Vero cell culture and reverse transcription-polymerase chain reaction assays. Three virus isolates were obtained from 34,567 field-collected Ae. albopictus, and all were identified as Cache Valley virus by molecular methods. Ae. albopictus (N = 3,138), collected in Mercer County from late July through early September 2011, also were retested for West Nile virus (WNV) by reverse transcription-polymerase chain reaction, and all were negative. These results corroborate previous findings showing that Ae. albopictus may occasionally acquire Cache Valley virus, a deer-associated arbovirus, in nature. In contrast, we did not detect WNV infection in Ae. albopictus despite concurrent WNV amplification in this region.