Tissue-specific targeting of DNA nanodevices in a multicellular living organism.
Tissue-specific targeting of DNA nanodevices in a multicellular living organism.
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DOI：
10.7554/elife.67830
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发表时间：
2021-07-28
期刊：
影响因子：
7.7
通讯作者：
Krishnan Y
中科院分区：
文献类型：
--
作者：
Chakraborty K;Anees P;Surana S;Martin S;Aburas J;Moutel S;Perez F;Koushika SP;Kratsios P;Krishnan Y
Nucleic acid nanodevices present great potential as agents for logic-based therapeutic intervention as well as in basic biology. Often, however, the disease targets that need corrective action are localized in specific organs, and thus realizing the full potential of DNA nanodevices also requires ways to target them to specific cell types in vivo.在这里，我们表明，通过利用内源性或合成的受体-配体相互作用并利用生物体呈现的生物屏障，我们可以将外部引入的DNA纳米装置以亚细胞精度靶向秀丽隐杆线虫的特定细胞类型。 DNA 纳米结构对体内组织特异性靶向的适应性显着扩展了其在生物医学应用和发现生物学中的效用。
Nucleic acid nanodevices present great potential as agents for logic-based therapeutic intervention as well as in basic biology. Often, however, the disease targets that need corrective action are localized in specific organs, and thus realizing the full potential of DNA nanodevices also requires ways to target them to specific cell types in vivo. Here, we show that by exploiting either endogenous or synthetic receptor-ligand interactions and leveraging the biological barriers presented by the organism, we can target extraneously introduced DNA nanodevices to specific cell types in Caenorhabditis elegans, with subcellular precision. The amenability of DNA nanostructures to tissue-specific targeting in vivo significantly expands their utility in biomedical applications and discovery biology.