Arabidopsis phosphoinositide-specific phospholipase C 4 negatively regulates seedling salt tolerance
Arabidopsis phosphoinositide-specific phospholipase C 4 negatively regulates seedling salt tolerance
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拟南芥磷酸肌醇特异性磷脂酶 C 4 负调节幼苗耐盐性
DOI：
10.1111/pce.12918
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发表时间：
2017-08-01
影响因子：
7.3
通讯作者：
Ren, Dongtao
中科院分区：
文献类型：
--
作者：
Xia, Keke;Wang, Bo;Ren, Dongtao
以往的生理和药理研究表明，磷脂酰肌醇特异性磷脂酶C（PI-PLC）的活性通过改变细胞内Ca ~（2+）浓度在调节植物盐胁迫反应中起重要作用。然而，需要确定参与这一过程的工厂PLC的各个成员。本文分析了AtPLC 4在拟南芥幼苗盐胁迫响应中的功能。与Col-0野生型幼苗相比，plc 4突变体幼苗对盐胁迫表现出低敏感性，并且盐低敏感表型可以通过天然启动子控制的AtPLC 4的表达来补充。AtPLC 4过表达的转基因幼苗（AtPLC 4 OE）表现出盐过敏表型，而其突变体表达失活的转基因幼苗（AtPLC 4 m OE）则不表现出这种表型。在plc 4突变体和AtPLC 4 OE幼苗中使用水母发光蛋白作为Ca 2+指示剂，AtPLC 4显示出正调节盐诱导的Ca 2+增加。EGTA部分挽救了AtPLC 4 OE幼苗的盐敏感表型。对盐敏感基因的分析表明，RD 29 B、MYB 15和ZAT 10的转录在plc 4突变体和AtPLC 4 OE幼苗中呈负调控。我们的研究结果表明，AtPLC 4负调控拟南芥幼苗的耐盐性，和Ca 2+可能参与调节这一过程。
Previous physiological and pharmacological studies have suggested that the activity of phosphoinositide-specific phospholipase C (PI-PLC) plays an important role in regulating plant salt stress responses by altering the intracellular Ca2+ concentration. However, the individual members of plant PLCs involved in this process need to be identified. Here, the function of AtPLC4 in the salt stress response of Arabidopsis seedlings was analysed. plc4 mutant seedlings showed hyposensitivity to salt stress compared with Col-0 wild-type seedlings, and the salt hyposensitive phenotype could be complemented by the expression of native promoter-controlled AtPLC4. Transgenic seedlings with AtPLC4 overexpression (AtPLC4 OE) exhibited a salt-hypersensitive phenotype, while transgenic seedlings with its inactive mutant expression (AtPLC4m OE) did not exhibit this phenotype. Using aequorin as a Ca2+ indicator in plc4 mutant and AtPLC4 OE seedlings, AtPLC4 was shown to positively regulate the salt-induced Ca2+ increase. The salt-hypersensitive phenotype of AtPLC4 OE seedlings was partially rescued by EGTA. An analysis of salt-responsive genes revealed that the transcription of RD29B, MYB15 and ZAT10 was inversely regulated in plc4 mutant and AtPLC4 OE seedlings. Our findings suggest that AtPLC4 negatively regulates the salt tolerance of Arabidopsis seedlings, and Ca2+ may be involved in regulating this process.