Quantitative trait locus mapping of airway responsiveness to chromosomes 6 and 7 in inbred mice
Quantitative trait locus mapping of airway responsiveness to chromosomes 6 and 7 in inbred mice
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DOI：
10.1152/ajplung.1999.277.6.l1118
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发表时间：
1999-12-01
影响因子：
4.9
通讯作者：
Drazen, JM
中科院分区：
文献类型：
--
作者：
De Sanctis, GT;Singer, JB;Drazen, JM
数量性状基因座（QTL）定位用于确定小鼠气道高反应性的染色体区域。在A/J和C3 H/HeJ亲本菌株以及来自这些菌株之间的杂交的后代中测量气道对乙酰甲胆碱的反应性。回交[（A/J x C3 H/HeJ）x C3 H/HeJ]后代（n = 137-227只供试标记的信息小鼠）的QTL作图揭示了与染色体6和7上的基因座的两个显著连锁。第6号染色体上的QTL证实了先前的报告，在相同的遗传背景下，在该区域的连锁;第二个QTL，在第7号染色体上，代表一个新的基因座。此外，我们还获得了17号染色体上连锁（比值比对数= 1.7）的暗示性证据，该染色体位于先前在A/J和C57 BL/6 J小鼠之间的杂交中确定的同一区域。A/J和C3 H/HeJ小鼠杂交中的气道反应性受到至少两个主要遗传基因座的控制，有证据表明第三个基因座先前曾与A/J和C57 BL/6 J杂交有关;这表明多种遗传因素对该表型的表达至关重要。
Quantitative trait locus (QTL) mapping was used to identify chromosomal regions contributing to airway hyperresponsiveness in mice. Airway responsiveness to methacholine was measured in A/J and C3H/HeJ parental strains as well as in progeny derived from crosses between these strains. QTL mapping of backcross [(A/J x C3H/HeJ) x C3H/HeJ] progeny (n = 137-227 informative mice for markers tested) revealed two significant Linkages to loci on chromosomes 6 and 7. The QTL on chromosome 6 confirms the previous report by others of a linkage in this region in the same genetic backgrounds; the second QTL, on chromosome 7, represents a novel locus. In addition, we obtained suggestive evidence for linkage (logarithm of odds ratio = 1.7) on chromosome 17, which lies in the same region previously identified in a cross between A/J and C57BL/6J mice. Airway responsiveness in a cross between A/J and C3H/HeJ mice is under the control of at least two major genetic loci, with evidence for a third locus that has been previously implicated in an A/J and C57BL/6J cross; this indicates that multiple genetic factors central the expression of this phenotype.