Comparison of Quantitative Buffy Coat technique (QBC) with Giemsa-stained thick film (GTF) for diagnosis of malaria
Comparison of Quantitative Buffy Coat technique (QBC) with Giemsa-stained thick film (GTF) for diagnosis of malaria
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DOI：
10.1016/j.parint.2007.06.007
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发表时间：
2007-12-01
影响因子：
1.9
通讯作者：
Nga, I. C.
中科院分区：
文献类型：
--
作者：
Adeoye, G. O.;Nga, I. C.
人们对使用荧光显微镜诊断疟疾重新产生了兴趣，促使在拉各斯的宜家综合医院(现为拉各斯州立大学教学医院)，将定量巴菲涂层技术(QBC)与旧标准Giemsa染色的厚血膜(GTF)进行了比较。采集了353名患者的血液样本，每个患者都进行了QBC和GTF技术检查。其中GTF阳性68例，QBC阳性70例，阳性率分别为19.3%和19.8%。以GTF为标准计算疟疾阳性率为19.3%。总体而言，在QBC和GTF阳性的患者中，女性的比例(58.6%和54.4%)高于男性(41.4%和45.6%)。QBC的总敏感性为55.9%，特异性为88.85%。与GTF比较，QBC的阳性预测值为54.3%，阴性预测值为89.4%，两者的符合率为82.4%。这些数值低于先前研究中报告的QBC数值。寄生虫密度低的标本，QBC的敏感性进一步降低(33.3%)。
The renewed interest in the use of fluorescent microscopy for malaria diagnosis prompted the comparison of Quantitative Buffy Coat technique (QBC) with the old standard Giemsa-stained Thick blood Film (GTF) in Ikeja General Hospital, now Lagos State University Teaching Hospital, in Lagos. Blood samples were collected from 353 patients, each examined with the QBC and GTF techniques. Of these, 68 were positive with GTF, 70 with QBC giving a positive rate of 19.3% and 19.8% respectively. The malaria positive rate was calculated as 19.3% using GTF as the standard. In general, females recorded higher percentages (58.6% and 54.4%) than males (41.4% and 45.6%) among those positive with QBC and GTF respectively. The overall sensitivity rate for QBC was 55.9% and the specificity was 88.85%. The positive and negative predictive values of QBC compared to GTF were 54.3%, 89.4% respectively while the concordance of the two techniques was 82.4%. These values were lower than those reported for QBC in previous studies. The sensitivity of QBC reduced further (33.3%) with samples having low parasite density (