Autologous tenocyte therapy using porcine-derived bioscaffolds for massive rotator cuff defect in rabbits
Autologous tenocyte therapy using porcine-derived bioscaffolds for massive rotator cuff defect in rabbits
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DOI：
10.1089/ten.2006.0266
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发表时间：
2007-07-01
影响因子：
--
通讯作者：
Zheng, Ming-Hao
中科院分区：
文献类型：
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作者：
Chen, Jimin M.;Willers, Craig;Zheng, Ming-Hao
大型回缩的肩袖肌腱撕裂在手术干预后无法修复或再次撕裂。本研究试图开发新的组织工程方法，利用接种肌腱细胞的生物支架对兔的大面积肩袖肌腱缺损进行肌腱重建。选择猪小肠黏膜下层（Restore）和I/III型胶原生物支架（ACI - Maix）作为自体肌腱细胞的生物支架载体。在植入前对自体肌腱细胞进行生物学特性研究。将接种肌腱细胞的生物支架作为插入移植物植入，以重建兔的大面积肩袖肌腱缺损。在制造缺损时切除的自体肩袖肌腱原位再植入作为阳性对照。在术后4周和8周对组织学结果进行分析并半定量分级。在4周时，两种接种肌腱细胞的生物支架都显示出与仅使用生物支架进行肩袖重建相似的炎症反应，且组织学分级不如对照修复组。然而，在8周时，两种接种肌腱细胞的生物支架的炎症反应明显少于单独使用生物支架的情况。此外，接种肌腱细胞的生物支架产生了与阳性对照相似的组织学外观。在基于胶原的生物支架上植入自体肌腱细胞比单独植入生物支架能使肩袖肌腱更好地愈合和重塑。
Large and retracted rotator cuff tendon tears fail to repair or retear after surgical intervention. This study attempted to develop novel tissue-engineering approaches using tenocyte-seeded bioscaffolds for tendon reconstruction of massive rotator cuff tendon defect in rabbits. Porcine small intestine submucosa (Restore) and type I/III collagen bioscaffold (ACI-Maix) were chosen as bioscaffold carriers for autologous tenocytes. Biological characterization of autologous tenocytes was conducted before the implantation. The tenocyte-seeded bioscaffolds were implanted as interposition grafts to reconstruct massive rotator cuff tendon defects in rabbits. In situ reimplantation of the autologous rotator cuff tendon, excised during defect creation, served as a positive control. Histological outcomes were analyzed and semi-quantitatively graded at 4 and 8 weeks after surgery. At 4 weeks, both tenocyte-seeded bioscaffolds displayed inflammatory reaction similar to bioscaffold-only cuff reconstruction, and the histological grading were inferior to control repair. However, at 8 weeks, inflammatory reaction of both tenocyte-seeded bioscaffolds were dramatically less than with bioscaffold alone. In addition, bioscaffolds seeded with tenocytes generated a histological appearance similar to that of the positive control. The implantation of autologous tenocytes on collagen-based bioscaffolds results in better rotator cuff tendon healing and remodeling than with the implantation of bioscaffold alone.