A novel functionally distinct subtype of striatal neuropeptide Y interneuron.
A novel functionally distinct subtype of striatal neuropeptide Y interneuron.
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DOI：
10.1523/jneurosci.2628-11.2011
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发表时间：
2011-11-16
期刊：
影响因子：
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通讯作者：
Tepper JM
中科院分区：
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作者：
Ibáñez-Sandoval O;Tecuapetla F;Unal B;Shah F;Koós T;Tepper JM
我们研究了转基因绿色荧光蛋白（GFP）-神经肽Y（NPY）报告小鼠中表达新纹状体神经肽Y（NPY）的中间神经元的特性。在体外全细胞记录和biocytin染色证明存在一类新的神经肽Y表达GABA能中间神经元，表现出电生理，神经化学和形态学特性显着不同，从先前描述的含神经肽Y，高原去极化低阈值尖峰（NPY-PLTS）中间神经元。新的NPY中间神经元类型（NPY-神经胶质样）不同于先前描述的NPY-PLTS中间神经元，表现出显着较低的输入电阻和超极化膜电位，定期，非调节尖峰响应于去极化电流注入和缺乏平台去极化或低阈值尖峰。NPY-神经胶质样中间神经元也很容易区分其密集的紧凑和高度分支的树突和局部轴突分支，形成鲜明对比的稀疏和扩展的轴突和树突分支的NPY-PLTS中间神经元的形态。此外，NPY-神经胶质样中间神经元不表达生长抑素或一氧化氮合酶的免疫荧光，这是无处不在的NPY-PLTS中间神经元。在与NPY-PLTS中间神经元的配对记录中，IPSP/Cs只能在棘状投射神经元（SPN）中很少被引出。相反，由NPY-神经胶质样中间神经元支配SPN神经的概率极高，突触非常可靠（未观察到失败），并且所产生的突触后反应是缓慢的GABAA受体介导的抑制性突触后电流（IPSC），该电流先前在纹状体中未描述，但已从皮层和海马中的NPY-GABA能神经胶质样中间神经元引起。这些特性表明，在新纹状体的整合特性中，NPY-PLTS和NPY-神经胶质样中间神经元具有独特和独特的作用。
We investigated the properties of neostriatal neuropeptide Y (NPY)-expressing interneurons in transgenic green fluorescent protein (GFP)-NPY reporter mice. In vitro whole cell recordings and biocytin staining demonstrated the existence of a novel class of neostriatal NPY-expressing GABAergic interneurons that exhibit electrophysiological, neurochemical and morphological properties strikingly different from those of previously described NPY-containing, plateau-depolarization low-threshold spike (NPY-PLTS) interneurons. The novel NPY interneuron type (NPY-neurogliaform) differed from previously described NPY-PLTS interneurons by exhibiting a significantly lower input resistance and hyperpolarized membrane potential, regular, non-accommodating spiking in response to depolarizing current injections and an absence of plateau depolarizations or low threshold spikes. NPY-neurogliaform interneurons were also easily distinguished morphologically by their dense compact and highly branched dendritic and local axonal arborizations that contrasted sharply with the sparse and extended axonal and dendritic arborizations of NPY-PLTS interneurons. Further, NPY-neurogliaform interneurons did not express immunofluorescence for somatostatin or nitric oxide synthase that was ubiquitous in NPY-PLTS interneurons. IPSP/Cs could only rarely be elicited in spiny projection neurons (SPN) in paired recordings with NPY-PLTS interneurons. In contrast, the probability of SPN innervation by NPY-neurogliaform interneurons was extremely high, the synapse very reliable (no failures were observed), and the resulting postsynaptic response was a slow, GABAA receptor-mediated inhibitory postsynaptic current (IPSC) that has not been previously described in striatum, but that has been elicited from NPY-GABAergic neurogliaform interneurons in cortex and hippocampus. These properties suggest unique and distinctive roles for NPY-PLTS and NPY-neurogliaform interneurons in the integrative properties of the neostriatum.