A cytorhabdovirus-based expression vector in Nilaparvata lugens, Laodelphax striatellus, and Sogatella furcifera
A cytorhabdovirus-based expression vector in Nilaparvata lugens, Laodelphax striatellus, and Sogatella furcifera
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褐飞虱、灰飞虱和白背飞虱中基于细胞弹状病毒的表达载体
DOI：
10.1016/j.ibmb.2021.103703
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发表时间：
2022
影响因子：
3.8
通讯作者：
Wang Xian-Bing
中科院分区：
文献类型：
--
作者：
Xu Wen-Ya;Fang Xiao-Dong;Cao Qing;Gao Qiang;Gao Dong-Min;Qiao Ji-Hui;Zang Ying;Xie Liang;Ding Zhi-Hang;Yang Yi-Zhou;Wang Ying;Wang Xian-Bing
褐飞虱（Nilaparvata lugens）、小褐飞虱（ladelphax striatellus）和白背飞虱（Sogatella furcifera）是有问题的害虫，它们通过韧皮部吸液和病毒传播造成严重的产量损失。大麦黄条纹花叶病毒（BYSMV）是一种植物细胞habdov病毒，已发展成为SBPHs和谷类植物的多功能表达平台。然而，基于重组BYSMV的生物安全过表达载体（rBYSMV）仍有待开发并应用于三种飞虱。通过显微注射rBYSMV感染的大麦叶片粗提物，我们发现rBYSMV能够感染SBPHs、BPHs和WBPHs。为了确保rBYSMV载体的生物安全性，我们通过删除辅助蛋白P3（一种假定的病毒运动蛋白）产生了rBYSMV突变体。正如预期的那样，所得到的突变体消除了大麦植株中的病毒全身感染，但对BYSMV在昆虫媒介中的感染性没有影响。随后，我们利用修饰后的rBYSMV载体在3种飞虱中过表达铁转运肽（ITP），揭示了ITP的潜在功能。总之，我们的研究结果为飞虱的功能基因组学研究提供了生物安全的过表达平台。
The brown planthopper (BPH,Nilaparvata lugens), the small brown planthopper (SBPH, Laodelphax striatellus), and the white-backed planthopper (WBPH,Sogatella furcifera) are problematic insect pests and cause severe yield losses through phloem sap-sucking and virus transmission.Barley yellow striate mosaic virus(BYSMV), a plant cytorhabdovirus, has been developed as versatile expression platforms in SBPHs and cereal plants. However, bio-safe overexpression vectors based on recombinant BYSMV (rBYSMV) remain to be developed and applied to the three kinds of planthoppers. Here, we found that rBYSMV was able to infect SBPHs, BPHs and WBPHs through microinjection with crude extracts from rBYSMV-infected barley leaves. To ensure bio-safety of the rBYSMV vectors, we generated an rBYSMV mutant by deleting the accessory protein P3, a putative viral movement protein. As expected, the resulting mutant abolished viral systemic infection in barley plants but had no effects on BYSMV infectivity in insect vectors. Subsequently, we used the modified rBYSMV vector to overexpress iron transport peptide (ITP) in the three kinds of planthoppers and revealed the potential functions of ITP. Overall, our results provide bio-safe overexpression platforms to facilitate functional genomics studies of planthoppers.