CRISPR-Induced TMPRSS2-ERG Gene Fusions in Mouse Prostate Organoids.
CRISPR-Induced TMPRSS2-ERG Gene Fusions in Mouse Prostate Organoids.
复制标题
DOI：
--
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
E. Driehuis;H. Clevers
中科院分区：
文献类型：
--
作者：
E. Driehuis;H. Clevers
TMPRSS2-ERG 融合是前列腺癌中常见的遗传事件。到目前为止，这种基因改变都是通过 ERG 过度表达来建模的。在这篇简短的通讯中，我们报告了通过基于 CRISPR/Cas9 的策略进行基因融合的小鼠前列腺类器官的创建。 TMPRSS2 和 ERG 的基因融合导致 ERG 过度表达。这种效应是雄激素受体介导的，因为通过雄激素受体拮抗剂尼鲁米特治疗，融合转录物的表达可以恢复到野生型ERG水平。
TMPRSS2-ERG fusions are common genetic events in prostate cancer. Until now, this genetic alteration was modelled by ERG overexpression. In this short communication, we report the creation of mouse prostate organoids that have undergone gene fusion through a CRISPR/Cas9-based strategy. The genetic fusion of TMPRSS2 and ERG results in ERG overexpression. This effect is androgen receptor-mediated, as expression of the fusion transcript can be restored to wildtype ERG levels by treatment with the androgen receptor antagonist Nilutamide.