Improved Storage Properties and Cellular Uptake of Casticin-Loaded Nanoemulsions Stabilized by Whey Protein-Lactose Conjugate.
Improved Storage Properties and Cellular Uptake of Casticin-Loaded Nanoemulsions Stabilized by Whey Protein-Lactose Conjugate.
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DOI：
10.3390/foods10071640
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发表时间：
2021-07-15
期刊：
影响因子：
--
通讯作者：
Jin W
中科院分区：
文献类型：
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作者：
Zhang B;Lei M;Huang W;Liu G;Jiang F;Peng D;Huang Q;Jin W
紫花牡荆素具有广泛的功能活性，但它在食品中的水溶性较差。在此，制备了一种由美拉德乳清分离蛋白共轭物（MWPI）稳定的纳米乳液来包封紫花牡荆素。这种平均直径为200纳米的纳米乳液能够负载700微克/克紫花牡荆素。在储存4周期间，MWPI稳定的纳米乳液比乳清分离蛋白（WPI）纳米乳液表现出更好的稳定性。研究了负载紫花牡荆素的纳米乳液对癌细胞的抑制作用以及细胞摄取过程。结果表明，负载紫花牡荆素的纳米乳液在HepG2和MCF - 7细胞中的抑制活性比游离的紫花牡荆素更好。纳米乳液的细胞摄取呈现时间依赖性。纳米乳液进入HepG2和MCF - 7细胞后，会定位在溶酶体中，而不是细胞核内。纳米乳液进入HepG2细胞的主要途径是胞饮作用，而进入MCF - 7细胞主要是通过网格蛋白介导的凹陷。这项工作意味着MWPI稳定的纳米乳液可作为一种有效的递送系统来负载紫花牡荆素，并有可能应用于食品和制药行业。
Casticin has wide-ranging functional activities, but its water solubility is poor in food products. Here, a nanoemulsion stabilized by Maillard whey protein isolate conjugates (MWPI) was fabricated to encapsulate casticin. The nanoemulsion, with an average diameter of 200 nm, possessed the capability to load 700 μg/g casticin. MWPI-stabilized nanoemulsion showed better stability than that of the WPI nanoemulsion during 4 weeks of storage. Both the inhibition effects of the casticin-loaded nanoemulsion on cancer cells and the process of cellular uptake were studied. Results revealed that the casticin-loaded nanoemulsion had better inhibitory activity in HepG2 and MCF-7 cells than free casticin. Cellular uptake of the nanoemulsion displayed a time-dependent manner. After the nanoemulsion passed into HepG2 and MCF-7 cells, it would locate in the lysosome but not in the nucleus. The main pathway for the nanoemulsion to enter HepG2 cells was pinocytosis, whereas, it entered MCF-7 predominantly through the clathrin-mediated pit. This work implies that MWPI-stabilized nanoemulsions could be utilized as an effective delivery system to load casticin and have the potential to be applied in the food and pharmaceutical industries.