Synergistic activity of melittin with mupirocin: A study against methicillin-resistant S. Aureus (MRSA) and methicillin-susceptible S. Aureus (MSSA) isolates
Synergistic activity of melittin with mupirocin: A study against methicillin-resistant S. Aureus (MRSA) and methicillin-susceptible S. Aureus (MSSA) isolates
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DOI：
10.1016/j.sjbs.2020.05.027
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发表时间：
2020-10-01
影响因子：
4.4
通讯作者：
Babaeekhou, Laleh
中科院分区：
文献类型：
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作者：
Alni, Reza Hakimi;Tavasoli, Fatemeh;Babaeekhou, Laleh
耐甲氧西林金黄色葡萄球菌（MRSA）生物膜与各种医院感染有关，受到学术研究的关注。目前的研究旨在确定蜂毒肽对浮游和生物膜形式的金黄色葡萄球菌的抗菌作用。在鉴定 MRSA 和 SCCmec 类型（使用 PCR 方法）后，通过微量肉汤稀释法测定蜂毒肽和莫匹罗星的最低抑菌浓度 (MIC)、最低杀菌浓度 (MBC) 和分数抑菌浓度指数 (FICi)。使用微量滴定板测试（MtP）和扫描电子显微镜（SEM）方法测定蜂毒肽抗生物膜活性。采用实时定量RT-PCR方法检测1/2 MIC蜂毒肽的群体感应抑制活性，并采用四唑比色法（MTT）检测蜂毒肽对Vero细胞的细胞毒性。我们的研究结果表明，蜂毒肽和莫匹罗星的MIC值的几何平均值分别为4.4和14.22μg/ml。蜂毒肽-莫匹罗星的 FICi 几何平均值均为 0.75。没有形成金黄色葡萄球菌生物膜，并且hld基因（作为生物膜调节剂）表达下调。蜂毒肽似乎可以通过降低 hld 表达来治疗金黄色葡萄球菌感染（尤其是 MRSA）。此外，莫匹罗星与蜂毒肽的协同生长抑制作用可被认为是治疗 MRSA 分离株的一种有前途的方法。 (C) 2020 年由 Elsevier B.V. 代表沙特国王大学出版。
Methicillin-Resistant Staphylococcus aureus (MRSA) biofilms are involved in various nosocomial infections, being in the limelight of academic research. The current study aimed to determine the antimicrobial effects of melittin on planktonic and biofilm forms of S. aureus. Following the identification of MRSA and SCCmec types (using PCR method), Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and fractional inhibitory concentration index (FICi), for melittin and mupirocin were determined by broth microdilution assay. Melittin anti-biofilm activity was determined, using a microtiter-plate test (MtP) and scanning electron microscope (SEM) methods. The quorum sensing inhibitory activity of 1/2 MIC melittin was examined using a quantitative real-time RT-PCR method, and melittin cytotoxicity on Vero cells was examined by tetrazolium-based colorimetric (MTT) test. The Results of our study showed that Geometric means of MIC values of the melittin and mupirocin were 4.4 and 14.22 mu g/ml respectively. The geometric mean of the FICi for both melittin-mupirocin was 0.75. No S. aureus biofilm was formed and hld gene (as a biofilm regulator) expression down-regulated. It seems that melittin can be useful in the treatment of S. aureus infections (especially MRSA) by reducing the hld expression. Furthermore, synergistic growth-inhibitory effects of mupirocin with melittin could be considered as a promising approach in the treatment of MRSA isolates. (C) 2020 Published by Elsevier B.V. on behalf of King Saud University.