Impact of Oral Hygiene Discontinuation on Supragingival and Salivary Microbiomes.
Impact of Oral Hygiene Discontinuation on Supragingival and Salivary Microbiomes.
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DOI：
10.1177/2380084417723625
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发表时间：
2018-01
影响因子：
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通讯作者：
Holmstrup P
中科院分区：
文献类型：
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作者：
Belstrøm D;Sembler-Møller ML;Grande MA;Kirkby N;Cotton SL;Paster BJ;Twetman S;Holmstrup P
本研究的目的是描述和比较停止口腔卫生10天期间的牙龈上和唾液中的微生物。我们检验了这样的假设，即唾液微生物区系的组成将反映与积累的生物膜形成和成熟相关的局部微生物变化。收集牙周上菌斑(n=145)和刺激性唾液(n=145)样本，记录29名口腔健康人在基线、停止口腔卫生时(第4、7、10天)和恢复口腔卫生后14天的菌斑和牙龈指数。通过下一代测序(使用下一代测序进行人类口腔微生物鉴定)和微生物群落图谱的比较来处理牙龈上和唾液中的微生物。在停止口腔卫生4天、7天和10天以及重新恢复口腔卫生后14天收集的牙龈上菌斑样本的微生物组成与基线样本有显著差异，细毛菌的相对丰度增加了3倍，链球菌的相对数量减少了2倍(调整后P<；0.01)。在唾液样本中，细毛菌的相对丰度在第7天明显增加(调整后P<；0.01)，但在恢复口腔卫生后第14天完全逆转。虽然唾液微生物区系对累积的局部生物膜形成具有抵抗力，但本研究的数据显示，尽管菌斑恢复到基线水平，但在恢复口腔卫生14天后，牙龈上微生物区系的组成变化并未逆转。(ClinicalTrials.gov UCPH_OI_002，NCT02913235)。知识转移声明：这项研究的数据显示，停止口腔卫生10天后，牙龈上微生物群的组成发生了变化，在恢复口腔卫生14天后，这种变化不会逆转。值得注意的是，停止口腔卫生与潜在致龋性细毛菌的相对丰度显著增加和链球菌种的减少有关。因此，这项研究的结果强调了有规律的口腔卫生在维持口腔动态平衡方面的必要性。
The purpose of the present study was to characterize and compare supragingival and salivary microbiotas during a 10-d period of oral hygiene discontinuation. We tested the hypothesis that the composition of the salivary microbiota will reflect local microbial changes associated with accumulated biofilm formation and maturation. Pooled supragingival plaque (n = 145) and stimulated saliva (n = 145) samples were collected and plaque and gingival indices were recorded from 29 orally healthy individuals at baseline, during oral hygiene discontinuation (days 4, 7, and 10), and 14 d after resumption of oral hygiene. Supragingival and salivary microbiotas were processed by next-generation sequencing (Human Oral Microbe Identification using Next Generation Sequencing) and microbial community profiles were compared. Microbial composition of supragingival plaque samples collected after 4, 7, and 10 d of oral hygiene discontinuation, as well as 14 d after reuptake of oral hygiene, differed significantly from baseline samples, by a 3-fold increase in relative abundance Leptotrichia species and a 2-fold decrease in Streptococcus species (adjusted P < 0.01). In saliva samples, a significant increase in relative abundance of Leptotrichia species (adjusted P < 0.01) was evident at day 7 but completely reversed 14 d after resumption of oral hygiene. While the salivary microbiota was resistant to accumulated local biofilm formation, data from this study showed that compositional changes of supragingival microbiotas were not reversed 14 d after resumption of oral hygiene, despite the restoration of plaque to baseline levels. (ClinicalTrials.gov UCPH_OI_002, NCT02913235). Knowledge Transfer Statement: Data from this study showed compositional changes of supragingival microbiotas as a consequence of a 10-d period of oral hygiene discontinuation, that was not reversed 14 d after resumption of oral hygiene. Notably, oral hygiene discontinuation was associated with a significant increase in relative abundance of potential cariogenic Leptotrichia species and a decrease in Streptococcus species. Thus, findings from this study highlight the necessity of regular oral hygiene in the maintenance of oral homeostasis.