Morphometric, histomorphometric, and microcomputed tomographic analysis of periodontal inflammatory lesions in a murine model
Morphometric, histomorphometric, and microcomputed tomographic analysis of periodontal inflammatory lesions in a murine model
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DOI：
10.1902/jop.2007.060320
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发表时间：
2007-06-01
影响因子：
4.3
通讯作者：
Amar, Salomon
中科院分区：
文献类型：
--
作者：
Li, Chung Hsing;Amar, Salomon
背景：牙龈卟啉单胞菌是慢性牙周炎的主要牙周病原体之一，是一种以牙周组织炎症和破坏为特征的常见感染性疾病。几种牙周炎的动物模型已经被用于牙周炎的研究。此外，多种方法也被应用于测量牙周炎模型中的牙槽骨丢失，包括组织形态计量学、形态计量学和放射学。本研究的目的是评估牙周炎后牙周炎性病变，并利用该模型比较三种评估牙槽骨丢失的方法。方法：12周龄雄性C57BL/6小鼠随机分为两组：48只感染牙周炎的C57BL/6小鼠和52只未治疗的对照组。牙周炎动物模型是用浸泡过牙龈假单胞菌的结扎线包裹左侧上颌第二磨牙，每隔一天更换一次结扎线。分别于结扎后第0、3、7、10天处死小鼠，每个时间点共12只实验组和13只对照组。结果：在整个实验期间，牙龈假单胞菌感染组与对照组相比，牙槽骨上皮生长抑制(P<；0.05)、炎症(P<；0.05)、牙槽骨丢失(P<；0.05)和破骨细胞活性(P<；0.05)均显著增加。所有这三种方法都能有效地评估牙槽骨丢失。结论：我们的结果表明，牙龈假单胞菌结扎诱导的小鼠模型具有足够的炎症反应，并显示出与其他牙周病模型相一致的牙周组织破坏。此外，使用本文中提出的三种牙槽骨分析中的任何一种，都可以准确地量化牙槽骨丢失。
Background: Porphyromonas gingivalis is recognized as one of the major periodontal pathogens in chronic periodontitis, a common infectious disease characterized by inflammation and destruction of periodontal tissues. Several animal models with P. gingivalis have been used in periodontitis studies. Additionally, multiple approaches have also been applied to measuring alveolar bone loss in periodontitis models, including histomorphometry, morphometry, and radiography. The aims of this study were to assess periodontal inflammatory lesions after P. gingivalis-induced periodontitis and use this model to compare three approaches for assessing alveolar bone loss.Methods: Twelve-week-old male C57BL/6 mice were divided into two groups: 48 P. gingivalis-infected and 52 untreated control mice. Periodontitis was induced by wrapping P. gingivalis-soaked ligatures around the left maxillary second molar and changing the ligatures every other day. Mice were euthanized on days 0, 3, 7, and 10 after ligature placement, for a total of 12 experimental and 13 control mice per time point. Epithelial downgrowth, inflammation, and osteoclast activity were evaluated; alveolar bone loss was determined by histomorphometry, morphometry, and microcomputed tomography.Results: The P. gingivalis-infected group showed significantly increased epithelial downgrowth (P < 0.05), inflammation (P < 0.05), alveolar bone loss (P < 0.05), and osteoclast activity (P < 0.05) throughout the experimental period compared to the controls. All three methods yielded efficient evaluation of alveolar bone loss.Conclusions: Our results show evidence that the P. gingivalis-soaked ligature-induced murine model mounts an adequate inflammatory response and exhibits periodontal tissue breakdown compatible with other models of periodontal disease. In addition, alveolar bone loss can accurately be quantified using any of the three alveolar bone analyses presented in this article.