Baseline Sensitivity and Resistance-Risk Assessment of Phytophthora capsici to Iprovalicarb
Baseline Sensitivity and Resistance-Risk Assessment of Phytophthora capsici to Iprovalicarb
复制标题
辣椒疫霉对异丙威的基线敏感性和耐药性风险评估
DOI：
10.1094/phyto-12-09-0351
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发表时间：
2010-11-01
期刊：
影响因子：
3.2
通讯作者：
Hao, Jianjun J.
中科院分区：
文献类型：
--
作者：
Lu, Xiao Hong;Zhu, Shu Sheng;Hao, Jianjun J.
异丙戊醇已用于防治辣椒疫霉（Phytophthora capsici），辣椒疫霉是许多经济上重要的作物的毁灭性病原体。为了评估杀菌剂抗性的风险，通过测量菌丝生长来检查158个辣椒疫霉分离物对异丙戊醇b的敏感性。50%菌丝生长抑制的有效浓度值在0.2042 - 0.5540 μ g/ml之间变化，平均值为0.3923（+/- 0.0552）μ g/ml，呈单峰分布。这是首次报道辣椒疫霉对异丙戊醇b高度耐药（耐药因子>100）。通过10次转移，菌株的抗性是稳定的，大多数抗性菌株具有相同的健身水平（菌丝生长，游动孢子生产，和毒力）作为其相应的父母，这表明异丙戊醇的抗性是独立于其他一般的生长性状。所有测试的羧酸酰胺（CAA）杀菌剂，包括异丙戊菌胺，氟吗啉，烯酰吗啉，双炔酰菌胺之间存在交叉抗性，但不与非CAA杀菌剂，包括嘧菌酯，百菌清，霜脲氰，烯菌唑，甲霜灵，苯酰菌胺。根据目前的结果，辣椒疫霉菌对CAA的抗性风险可能是中等的，应考虑抗性管理。
Iprovalicarb has been used to control Phytophthora capsici, a devastating pathogen of many economically important crops. To evaluate the risk of fungicide resistance, 158 isolates of P. capsici were examined for sensitivity to iprovalicarb by measuring mycelial growth. Values of effective concentrations for 50% mycelial growth inhibition varied from 0.2042 to 0.5540 mu g/ml and averaged 0.3923 (+/- 0.0552) mu g/ml, with a unimodal distribution. This is the first report of P. capsici isolates highly resistant to iprovalicarb (resistance factor >100). Resistance of the isolates was stable through 10 transfers on iprovalicarb-free medium, and most resistant isolates had the same level of fitness (mycelial growth, zoospore production, and virulence) as their corresponding parents, indicating that iprovalicarb resistance was independent from other general growth characters. There was cross-resistance among all tested carboxylic acid amide (CAA) fungicides, including iprovalicarb, flumorph, dimethomorph, and mandipropamid, but not with non-CAA fungicides, including azoxystrobin, chlorothalonil, cymoxanil, etridiazole, metalaxyl, and zoxamide. Based on the present results, resistance risk of P capsici to CAAs could be moderate and resistance management should be considered.