Anaerobiosis and low specific growth rates enhance hemolysin BL production by Bacillus cereus F4430/73
Anaerobiosis and low specific growth rates enhance hemolysin BL production by Bacillus cereus F4430/73
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DOI：
10.1007/s00203-004-0688-y
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发表时间：
2004-09-01
影响因子：
2.8
通讯作者：
Schmitt, P
中科院分区：
文献类型：
--
作者：
Duport, C;Thomassin, S;Schmitt, P
蜡状芽孢杆菌F4430/73在MOD培养基中厌氧生长时产生最高水平的溶血素BL（HBL）。在恒化器中以低比生长速率（0.1-0.2 h（-1））生长的厌氧细胞表达的HBL比以更快的生长速率生长的细胞多7倍。在0.2 h（-1）时，90 mM葡萄糖的存在导致HBL产生的抑制。发现葡萄糖在mRNA水平上抑制HBL诱导，表明分解代谢产物抑制在HBL调节中的潜在参与。基于这些数据，这表明，生长速度可能是HBL的分解代谢调节的效应。
Bacillus cereus F4430/73 produced the highest levels of hemolysin BL (HBL) when grown under anaerobiosis in MOD medium. Anaerobic cells grown in a chemostat at low specific growth rate (0.1-0.2 h(-1)) expressed up to sevenfold more HBL than did cells held at a faster growth rate. At 0.2 h(-1), the presence of 90 mM glucose resulted in inhibition of HBL production. Glucose was found to repress HBL induction at the mRNA level, indicating the potential involvement of catabolite repression in the regulation of HBL. Based on these data, it is suggested that growth rate could be an effector of catabolite regulation of HBL.