Expression of KL-6 mucin, a human MUC1 mucin, in intrahepatic cholangiocarcinoma and its potential involvement in tumor cell adhesion and invasion
Expression of KL-6 mucin, a human MUC1 mucin, in intrahepatic cholangiocarcinoma and its potential involvement in tumor cell adhesion and invasion
复制标题
KL-6粘蛋白（一种人MUC1粘蛋白）在肝内胆管癌中的表达及其与肿瘤细胞粘附和侵袭的潜在参与
DOI：
10.1016/j.lfs.2009.07.004
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发表时间：
2009
期刊：
影响因子：
6.1
通讯作者：
Tang W.
中科院分区：
文献类型：
--
作者：
Xu HL;Inagaki Y;Seyama Y;Sugawara Y;Kokudo N;Nakata M;Wang FS;Tang W.
KL-6粘蛋白的异常表达与多种肿瘤的恶性行为有关。为探讨粘蛋白KL-6在肝内胆管细胞癌（intrhepatic cholangiocarcinoma，CC）中的表达及其在肿瘤进展中的意义，采用免疫组织化学方法检测21例CC、12例肝细胞癌和78例肝细胞癌（hepatocellular carcinoma，HCC）中KL-6粘蛋白的表达。用MTT法检测衣霉素和苄基-α-N-乙酰半乳糖胺（BAG）对CC细胞增殖的影响。免疫细胞化学染色和Western blotting检测衣霉素或BAG处理后KL-6粘蛋白的表达。结果：经衣霉素或BAG处理后，所有CC组织和cHCC-CC组织中的CC区均可见KL-6粘蛋白染色阳性。免疫细胞化学染色和蛋白质印迹显示，KL-6粘蛋白表达显着减少后，两种抑制剂处理。两种抑制剂处理后细胞粘附性显著降低，而BAG处理后细胞侵袭能力显著降低，但衣霉素处理后细胞侵袭能力无显著降低。针对KL-6粘蛋白糖基化的治疗策略可能有助于控制CC的侵袭行为。
AIMSAberrant expressions of KL-6 mucin were proved to be associated with worse tumor behaviors of many carcinomas. This study was to evaluate the expression KL-6 mucin, a human MUC1 mucin, in intrahepatic cholangiocarcinoma (CC) and its significance in tumor progression.MAIN METHODSKL-6 mucin expressions in 21 patients with CC, 12 with combined hepatocellular and cholangiocarcinoma (cHCC-CC), and 78 with hepatocellular carcinoma (HCC) were detected by immunohistochemical staining. The effects of two glycosylation inhibitors (tunicamycin and benzyl-alpha-N-acetylgalactosamine (BAG)) on CC cell proliferations were assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assays. KL-6 mucin expressions were detected by immunocytochemical staining and western blotting after tunicamycin or BAG treatment. Cell adhesive and invasive properties were evaluated by adhesion tests and transwell chamber assays after tunicamycin or BAG treatment.KEY FINDINGSPositive KL-6 mucin staining was observed in all CC tissues and CC areas of cHCC-CC tissues. Immunocytochemical staining and western blotting showed that KL-6 mucin expressions were significantly reduced after both inhibitors treatment. Cell adhesive properties were significantly decreased after both inhibitors treatment, while cell invasive abilities were significantly decreased after BAG but not tunicamycin treatment.SIGNIFICANCEThis study indicated that KL-6 mucin might be a specific tumor target for CC. Therapeutic strategies that target glycosylation of KL-6 mucin may be useful to control aggressive behaviors of CC.