Microarray profiling reveals microRNAs involving soybean resistance to Phytophthora sojae
Microarray profiling reveals microRNAs involving soybean resistance to Phytophthora sojae
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DOI：
10.1139/g11-050
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发表时间：
2011-11-01
期刊：
影响因子：
3.1
通讯作者：
Dou, Dao-Long
中科院分区：
文献类型：
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作者：
Guo, Na;Ye, Wen-Wu;Dou, Dao-Long
微小RNA（miRNAs）是一类小分子非编码RNA，可作为植物免疫系统中的一类转录后调节因子。然而，对于它们在植物对卵菌病原体的免疫反应中的作用知之甚少。为了鉴定参与大豆对大豆疫霉反应的miRNAs，我们通过芯片分析检测了三个大豆品种（感病品种Williams、数量抗性品种Conrad和质量抗性品种Williams 82）在受到大豆疫霉侵染时miRNAs的表达模式。许多miRNAs的表达在侵染时和（或）不同基因型中发生了显著改变。一些miRNAs的实时定量PCR数据进一步证实了芯片结果。对所选miRNAs及其靶基因表达数据集的比较分析揭示了许多相互表达的miRNA - 靶标对，这可能表明miRNAs与蛋白质编码基因之间存在反馈回路。这些结果可为进一步深入研究参与大豆对大豆疫霉抗性的miRNAs奠定基础。
MicroRNAs (miRNAs), a group of small noncoding RNAs, may serve as a class of post-transcriptional regulators in plant immune systems. Nevertheless, little is known about their roles in plant immune response to the oomycete pathogens. To identify miRNAs involved in the response of soybean to Phytophthora sojae, we examined expressional patterns of miRNAs upon infection by P sojae by microarray analysis in three soybean cultivars: Williams (susceptible), Conrad (quantitative resistance), and Williams 82 (qualitative resistance). Expression of a number of miRNAs was significantly altered upon infection and (or) in the different genotypes. qRT-PCR data with some miRNAs further confirmed the microarray results. Comparative analysis of the selected miRNAs and their targeted gene expression datasets uncovered many reciprocally expressed miRNA target pairs, which could proposed a feedback circuit between miRNA(s) and protein-coding genes. These results may serve as a basis for further in-depth studies of miRNAs involved in soybean resistance to P. sojae.