Bactericidal and Detoxification Effects of TiO2 Thin Film Photocatalysts
Bactericidal and Detoxification Effects of TiO2 Thin Film Photocatalysts
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DOI：
10.1021/es970860o
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发表时间：
1998-01
影响因子：
11.4
通讯作者：
K. Sunada;Yoshihiko Kikuchi;K. Hashimoto;A. Fujishima
中科院分区：
文献类型：
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作者：
K. Sunada;Yoshihiko Kikuchi;K. Hashimoto;A. Fujishima
为了考察透明二氧化钛薄膜的抗菌特性，研究了光催化降解大肠杆菌热源成分内毒素及其杀菌活性。从异丙醇钛溶液中制备二氧化钛薄膜，在500℃下热处理，根据营养琼脂平板上形成的活细胞数计算存活率，评估其对大肠杆菌细胞的杀菌活性。细菌内毒素浓度测定采用凝胶试验法。在紫外光照射下，用TiO2光催化剂杀灭大肠杆菌细胞，也能有效地降解细胞中的内毒素。这一结果表明，TiO2光催化剂具有杀菌活性和分解内毒素的活性(即解毒活性)。二氧化钛薄膜的杀菌作用既是由于灭活了细菌的活性，又是由于它对细菌的破坏作用。
To examine the special features of the antibacterial effect for a thin transparent titanium dioxide (TiO2) film, the photocatalytic degradation of endotoxin, which is a pyrogenic constituent of Escherichia coli (E. coli), as well as its bactericidal activity, was investigated. The TiO2 films were prepared from titanium isopropoxide solution, annealing at 500 °C. The bactericidal activity for E. coli cells was estimated by survival ratio calculated from the number of viable cells which form colonies on the nutrient agar plates. The endotoxin concentration was determined by the Limulus tests. When E. coli cells were killed by the TiO2 photocatalyst under UV irradiation, the endotoxin from the cells was also degraded efficiently. This result shows that the TiO2 photocatalyst has both bactericidal activity and decomposing activity for the endotoxin (i.e., detoxifying activity). The bactericidal effect of the TiO2 thin film results from both inactivating the viability of the bacteria and the destruction of the...