Simultaneous determination of unbound cefoperazone in rat blood and brain using microdialysis
Simultaneous determination of unbound cefoperazone in rat blood and brain using microdialysis
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DOI：
10.1211/0022357001774859
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发表时间：
2000-08-01
影响因子：
3.3
通讯作者：
Tsai, TH
中科院分区：
文献类型：
--
作者：
Chang, YL;Chou, MH;Tsai, TH
采用微透析技术建立了一种同时测定大鼠血液和脑组织中游离头孢哌酮浓度的微柱高效液相色谱法。然后通过股静脉注射。收集血液和脑透析液，并用含有甲醇-100 mM三磷酸（30：70，v/v，pH 5.5）的移动的流动相洗脱。紫外检测器的波长设定为254 nm。头孢哌酮的检测限为20 ng mL（-1），头孢哌酮在10 min内达到等度分离，在0.05 ~ 10 μ g mL（-1）范围内，批内和批间分析的准确度和精密度均小于或等于10%，头孢哌酮在大鼠脑和血液中的浓度曲线下面积之比约为7.8%。结论：头孢哌酮具有穿透血脑屏障的能力。
A sensitive microbore HPLC method was developed for the simultaneous determination of unbound cefoperazone in rat blood and brain using microdialysis.Two microdialysis probes were inserted into the jugular vein/right atrium and brain striatum of Sprague-Dawley rats, Cefoperazone (50 mg kg(-1), i.v.) was then administered via the femoral vein. Blood and brain dialysates were collected and eluted with a mobile phase containing methanol-100 mM monosodium phosphoric acid (30:70, v/v, pH 5.5). The wavelength of the UV detector was set at 254 nm. The detection limit of cefoperazone was 20 ngmL(-1) Isocratic separation of cefoperazone was achieved within 10 min. The intra- and inter-assay accuracy and precision of the analyses were less than or equal to 10% in the range of 0.05-10 mu g mL(-1).The ratio of the area under the concentration curve of cefoperazone in rat brain and blood was estimated to be about 7.8%. It is concluded that cefoperazone is capable of penetrating the blood-brain barrier.