Distribution of O-GlcNAc transferase in the rat pancreas
Distribution of O-GlcNAc transferase in the rat pancreas
复制标题
O-GlcNAc转移酶在大鼠胰腺中的分布
DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
H. Hirano
中科院分区：
文献类型：
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作者：
Y. Akimoto;G. Hart;H. Hirano
O−GlcNAc 转移酶 (OGT) 催化 N−乙酰氨基葡萄糖单糖与细胞内蛋白质丝氨酸或苏氨酸残基的羟基结合，可能在己糖胺途径中发挥重要作用。我们检查了 OGT 蛋白在大鼠胰腺中的定位。使用针对 OGT 的抗体进行免疫荧光染色，对外分泌腺泡细胞和内分泌胰岛细胞进行染色。腺泡细胞核和酶原颗粒区域被强烈染色。在胰岛中，特别是在 A 细胞中，观察到抗 OGT 抗体的强烈染色。免疫电镜显示 OGT 仅限于细胞核的常染色质以及外分泌腺泡细胞和内分泌胰岛细胞的分泌颗粒周围。这些结果表明OGT参与转录和颗粒分泌的调节。因此，O-GlcNAc 酰化蛋白可能是胰腺葡萄糖传感机制的重要组成部分。
O−GlcNAc transferase(OGT) catalyzes the attachment of N−acetylglucosamine monosaccharides to the hydroxyl group of serine or threonine residues of intracellular proteins and may play an important role in the hexosamine pathway. We examined the localization of OGT protein in the rat pancreas. Immunofluorescence staining with antibody raised against OGT stained both the exocrine acinar cells and endocrine islet cells. The acinar cell nucleus and the zymogen granule region were intensely stained. In the islets of Langerhans, especially in the A cells, intense staining with anti−OGT antibody was observed. Immuno−electron microscopy showed the OGT to be restricted to the euchromatin of the nucleus and around the secretory granules of exocrine acinar cells and endocrine islet cells. These results suggest that OGT is involved in the regulation of transcription and of granular secretion. Thus, O−GlcNAcylated protein(s) may be an important component of the glucose−sensing mechanism in the pancreas.
DOI：
10.1016/s0021-9258(17)43295-9
发表时间：
1984-03
期刊：
The Journal of biological chemistry
影响因子：
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作者：
C R Torres;G. W. Hart
通讯作者：
C R Torres;G. W. Hart