Identification of geranylgeranyl-modified proteins in HeLa cells.
Identification of geranylgeranyl-modified proteins in HeLa cells.
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DOI：
10.1126/science.2296721
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发表时间：
1990-01
期刊：
影响因子：
56.9
通讯作者：
C. Farnsworth;Michael H. Gelb;J. A. Glomset
中科院分区：
文献类型：
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作者：
C. Farnsworth;Michael H. Gelb;J. A. Glomset
以前的研究表明，动物细胞含有类异戊二烯修饰的蛋白质，其中一种蛋白质，核纤层蛋白B，含有一个硫醚连接的法呢基，连接到半胱氨酸。在本研究中，一种新的类异戊二烯修饰被确定与[3H]甲羟戊酸标记的HeLa细胞和分析总细胞蛋白的蛋白水解酶。从这些放射性物质中提纯放射性碎片，并用雷尼镍处理。通过气液色谱法（GC）和质谱法（MS）分析释放的标记物质。这种方法表明，全反式香叶基香叶基是一个主要的类异戊二烯修饰。
Previous studies have shown that animal cells contain isoprenoid-modified proteins and that one of these proteins, lamin B, contains a thioether-linked farnesyl group that is attached to cysteine. In the present study, a novel isoprenoid-modification was identified by labeling HeLa cells with [3H]mevalonic acid and analyzing proteolytic digests of the total cell protein. Radioactive fragments were purified from these digests and treated with Raney nickel. The released, labeled material was analyzed by gas-liquid chromatography (GC) and mass spectrometry (MS). This approach revealed that an all-trans geranylgeranyl group was a major isoprenoid modification.