Mechanistic aspects of green tea as a cancer preventive: effect of components on human stomach cancer cell lines.
Mechanistic aspects of green tea as a cancer preventive: effect of components on human stomach cancer cell lines.
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DOI：
10.1111/j.1349-7006.1999.tb00808.x
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发表时间：
1999-07
期刊：
影响因子：
--
通讯作者：
Fujiki H
中科院分区：
文献类型：
--
作者：
Okabe S;Ochiai Y;Aida M;Park K;Kim SJ;Nomura T;Suganuma M;Fujiki H
现在人们普遍认为 (-)-表没食子儿茶素没食子酸酯 (EGCG) 可以抑制啮齿类动物消化道的致癌作用。为了了解抗癌机制，我们首先研究了 EGCG 对首尔国立大学建立的人胃癌细胞系（SNU 细胞系）的生长抑制作用。研究了 EGCG 对[3H]胸苷掺入八种 SNU 细胞系的抑制作用，以及与转化生长因子-β (TGF-β) 反应性的关系。绿茶和红茶中提取的各种茶多酚可诱导人胃癌细胞系 KATO III 的生长抑制和凋亡，并抑制细胞中肿瘤坏死因子-α (TNF-α) 的释放，其顺序依次为 (-)-表儿茶素没食子酸酯 (ECG)、EGCG、(-)-表没食子儿茶素 (EGC)、茶黄素 (TF) 和 (-)-表儿茶素 (EC)。此外，我们证明 EGCG 抑制 KATO III 细胞中 TNF-α 基因表达，以及冈田酸诱导的 AP-1 和 NF-κB 激活。 KATO III 细胞和小鼠成纤维细胞系 BALB/3T3 之间 EGCG 对 AP-1 和 NF-κB 与 DNA 结合的抑制效力不同。因此，EGCG和其他茶多酚可能与各种细胞核中除AP-1和NF-κB外的各种转录因子相互作用，从而抑制TNF-α基因表达和TNF-α释放。
It is now well accepted that (–)‐epigallocatechin gallate (EGCG) inhibits carcinogenesis in the digestive tract in rodents. To understand the mechanisms of anticarcinogenesis, we first studied growth inhibition by EGCG in human stomach cancer cell lines established at Seoul National University(SNU cell lines). Inhibition by EGCG of [3H]thymidine incorporation into eight SNU celllines was examined, in relation to transforming growth factor‐β (TGF‐β) responsiveness. Various tea polyphenols derived from green tea and black tea induced growth inhibition and apoptosis of human stomach cancer cell line KATO III, and inhibition of tumor necrosis factor‐α (TNF‐α)release from the cells, in the order of (–)‐epicatechin gallate (ECG), EGCG, (–)‐epigallocatechin(EGC), teaflavins (TF) and (–)‐epicatechin (EC). In addition, we demonstrated that EGCG inhibited TNF‐α gene expression in KATO III cells, as well as okadaic acid‐induced AP‐1 and NF‐κB activation. The inhibitory potencies of EGCG for AP‐1 and NF‐κB binding to DNA were different between KATO III cells and mouse fibroblast cell line BALB/3T3. Thus, EGCG and other teapolyphenols may interact with various transcription factors, in addition to AP‐1 and NF‐κB, in nuclei of various cells, resulting in inhibition of TNF‐α gene expression and TNF‐α release.
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影响因子：
6.1
作者：
Fujiki, H;Suganuma, M;Sakai, Y
通讯作者：
Sakai, Y
DOI：
10.1073/pnas.91.19.8772
发表时间：
1994-09-13
影响因子：
11.1
作者：
PARK, KC;KIM, SJ;SPORN, MB
通讯作者：
SPORN, MB
影响因子：
3.6
作者：
SUGANUMA, M;OKABE, S;FUJIKI, H
通讯作者：
FUJIKI, H
影响因子：
4.7
作者：
Sueoka, N;Sueoka, E;Fujiki, H
通讯作者：
Fujiki, H
影响因子：
5.5
作者：
NEWELL, CL;DEISSEROTH, AB;LOPEZBERESTEIN, G
通讯作者：
LOPEZBERESTEIN, G