RNA interference in mammalian cells by siRNAs modified with morpholino nucleoside analogues
RNA interference in mammalian cells by siRNAs modified with morpholino nucleoside analogues
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吗啉代核苷类似物修饰的 siRNA 对哺乳动物细胞中的 RNA 干扰
DOI：
10.1016/j.bmc.2009.02.001
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发表时间：
2009-03-15
影响因子：
3.5
通讯作者：
Jiang, Yuyang
中科院分区：
文献类型：
--
作者：
Zhang, Nan;Tan, Chunyan;Jiang, Yuyang
合成了吗啉核苷类似物修饰的siRNAs，并对其生物学特性进行了详细的研究。修饰后的siRNAs的基因沉默能力与Modi的位置有关。阳离子，其中一些似乎比天然的siRNA更有效。30端修饰显著提高了血清中siRNAs的稳定性。剂量-反应和时间-过程实验表明，经过30端修饰的siRNAs在较低浓度和较长作用时间下具有较强的基因沉默活性。这些良好的性质使吗啉修饰的siRNA在治疗应用中成为潜在的有用工具。(C)2009爱思唯尔有限公司。保留所有权利。
siRNAs modified with morpholino nucleoside analogues were synthesized and their biological properties were examined in details. The gene silence abilities of modified siRNAs were correlated to the positions of the modi. cations, some of which appeared to be more potent than the native siRNA. The 30-end modification improved the stability of siRNAs in serum significantly. Furthermore, the dose-response and time-course experiments demonstrated that the siRNAs with 30-end modification have potent gene silence activity at lower concentration and prolonged action time. These favorable properties make the morpholino modified siRNA a potentially useful tool in therapeutic applications. (C) 2009 Elsevier Ltd. All rights reserved.